| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NSMCE3 |
| Uniprot No | Q96MG7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-304aa |
| 氨基酸序列 | MLQKPRNRGRSGGQAERDRDWSHSGNPGASRAGEDARVLRDGFAEEAPSTSRGPGGSQGSQGPSPQGARRAQAAPAVGPRSQKQLELKVSELVQFLLIKDQKKIPIKRADILKHVIGDYKDIFPDLFKRAAERLQYVFGYKLVELEPKSNTYILINTLEPVEEDAEMRGDQGTPTTGLLMIVLGLIFMKGNTIKETEAWDFLRRLGVYPTKKHLIFGDPKKLITEDFVRQRYLEYRRIPHTDPVDYEFQWGPRTNLETSKMKVLKFVAKVHNQDPKDWPAQYCEALADEENRARPQPSGPAPSS |
| 预测分子量 | 38.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与NSMCE3重组蛋白相关的模拟参考文献(注：文献信息为示例性模拟，实际文献请通过学术数据库检索)：
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1. **文献名称**: *Structural insights into the SMC5/6 complex through NSMCE3 subunit analysis*
**作者**: Li et al.
**摘要**: 本研究通过重组表达NSMCE3蛋白，结合冷冻电镜技术解析了其与SMC5/6复合体的相互作用界面，揭示了NSMCE3在维持复合体稳定性及DNA损伤修复中的关键作用。
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2. **文献名称**: *Functional characterization of recombinant NSMCE3 in DNA crosslink repair*
**作者**: Garcia-Santos & Patel
**摘要**: 利用大肠杆菌系统表达纯化NSMCE3重组蛋白，发现其通过促进SMC5/6复合体的ATP酶活性，调控同源重组修复途径，对顺铂诱导的DNA交联损伤修复至关重要。
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3. **文献名称**: *NSMCE3 knockout and reconstitution assays define its role in telomere maintenance*
**作者**: Kimura et al.
**摘要**: 通过体外重组NSMCE3蛋白回补实验，证明该蛋白通过抑制端粒异常融合，维持基因组完整性，其功能缺失导致染色体末端保护缺陷。
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4. **文献名称**: *Biochemical reconstitution of NSMCE3-dependent SUMOylation in vitro*
**作者**: Wagner & Dantzer
**摘要**: 开发了基于重组NSMCE3蛋白的体外泛素化体系，阐明其作为E3连接酶辅助因子，在DNA损伤位点招募SUMO修饰酶的作用机制。
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**提示**：实际研究中建议通过PubMed或Web of Science检索关键词“NSMCE3 recombinant”“SMC5/6 complex”等获取最新文献。
**Background of NSMCE3 Recombinant Protein**
NSMCE3 (Structural Maintenance of Chromosomes 5/6 Complex Subunit E3), also known as MMS21. is a critical component of the SMC5/6 (Structural Maintenance of Chromosomes 5/6) complex, a conserved multi-protein assembly essential for maintaining genomic stability. The SMC5/6 complex plays pivotal roles in DNA repair, homologous recombination, chromosome segregation, and telomere maintenance. NSMCE3/MMS21 contributes to these functions primarily through its SUMO (Small Ubiquitin-like Modifier) ligase activity, which facilitates post-translational modification of target proteins involved in DNA damage response and chromatin remodeling.
Recombinant NSMCE3 protein is engineered to study its biochemical properties, interactions, and mechanisms in vitro or in cellular models. It is typically produced using expression systems like *E. coli* or mammalian cells, followed by purification via affinity tags (e.g., His-tag) to ensure high purity and functionality. The recombinant form retains the conserved C-terminal RING domain responsible for SUMOylation activity, enabling researchers to dissect its role in DNA repair pathways, such as resolving replication stress or mitigating oxidative damage.
Studies leveraging NSMCE3 recombinant protein have advanced understanding of diseases linked to SMC5/6 dysfunction, including cancer and genetic disorders. For instance, mutations in NSMCE3 are associated with genomic instability and increased cancer susceptibility. Additionally, its SUMO ligase activity is explored for therapeutic targeting, as aberrant SUMOylation is implicated in tumor progression. By enabling precise biochemical assays and structural studies, recombinant NSMCE3 serves as a vital tool for unraveling SMC5/6 complex dynamics and developing strategies to modulate its activity in disease contexts.
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