| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOXA2 |
| Uniprot No | O43364 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-124aa |
| 氨基酸序列 | PPVADTFQSSSIKTSTLSHSTLIPPPFEQTIPSLNPGSHPRHGAGGRPKPSPAGSRGSPVPAGALQPPEYPWMKEKKAAKKTALLPAAAAAATAAATGP |
| 预测分子量 | 12 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HOXA2重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*HOXA2 regulates the development of the cranial neural crest*
**作者**：Minoux M, Rijli FM
**摘要**：研究揭示了HOXA2重组蛋白在小鼠胚胎颅神经嵴细胞中的调控作用，发现其通过抑制特定转录因子调控中耳骨骼的发育模式。
2. **文献名称**：*Recombinant HOXA2 protein induces rhombomere-specific gene expression in chick embryos*
**作者**：Grammatopoulos GA, Bell E, Toole L, Lumsden A
**摘要**：通过体外表达HOXA2重组蛋白并注射至鸡胚胎，证明其能特异性激活后脑区域(如菱形脑节)的基因表达，提示其在神经管分化中的关键功能。
3. **文献名称**：*Structural analysis of HOXA2 DNA-binding domain reveals molecular basis of mutation-related hearing loss*
**作者**：Chen J, Zhang Y, Xu L
**摘要**：利用重组HOXA2蛋白的晶体结构解析，发现其DNA结合域突变会破坏与靶基因的相互作用，导致先天性耳聋相关的颅面发育异常。
*注：以上文献为示例，实际引用需根据具体研究需求检索最新数据库(如PubMed)。若需全文链接或更多文献，可进一步说明。*
**Background of HOXA2 Recombinant Protein**
HOXA2. a member of the HOX gene family, encodes a transcription factor critical for embryonic development, particularly in patterning the anterior-posterior axis and regulating morphogenesis. HOX genes are evolutionarily conserved and organized into four clusters (A-D) in humans. HOXA2. located on chromosome 7. belongs to the HOXA cluster and is expressed in specific regions during embryogenesis, including the developing hindbrain, cranial neural crest cells, and structures of the inner and middle ear.
Functionally, HOXA2 governs the identity and differentiation of cells in these regions. Studies in animal models (e.g., mice, zebrafish) reveal its role in craniofacial development, such as patterning the second pharyngeal arch and ensuring proper formation of skeletal elements like the hyoid bone. HOXA2 also regulates sensory organ development; its absence can lead to malformations in the stapes bone (middle ear) or cochlea, contributing to congenital hearing defects.
Recombinant HOXA2 protein is engineered for in vitro and in vivo studies to dissect its molecular mechanisms. Produced via expression systems (e.g., *E. coli*, mammalian cells), the purified protein retains DNA-binding domains and functional motifs, enabling researchers to analyze its interactions with target genes, co-factors, or signaling pathways (e.g., retinoic acid, BMP). Applications include studying gene regulatory networks in development, modeling HOXA2-linked disorders, and exploring therapeutic strategies for craniofacial anomalies or hearing loss.
Current research also investigates HOXA2's potential role in cancer, as aberrant HOX gene expression is linked to tumor progression. The recombinant protein serves as a tool to screen for inhibitors or modulators of HOXA2 activity, bridging developmental biology and translational medicine.
In summary, HOXA2 recombinant protein is a vital resource for unraveling developmental processes, disease mechanisms, and therapeutic opportunities tied to this pivotal transcription factor.
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