| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ER; ESR1; Era; ESRA |
| Entrez GeneID | 2099 |
| clone | 2B7 |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ER-alpha (410-592aa) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于ER-alpha抗体的参考文献示例(信息基于公开文献整理，非实时更新)：
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1. **文献名称**：*"Comparison of ER-alpha Antibody Clones for Immunohistochemical Detection of Estrogen Receptor in Breast Cancer"*
**作者**：Allred DC et al.
**摘要**：该研究比较了多种ER-alpha抗体克隆(如1D5、SP1、6F11)在乳腺癌组织中的检测性能，发现不同克隆的敏感性和特异性存在差异，建议结合临床病理特征优化抗体选择。
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2. **文献名称**：*"Validation of a Novel ER-alpha Monoclonal Antibody for Molecular Subtyping of Luminal Breast Cancers"*
**作者**：Hammond MEH et al.
**摘要**：研究验证了一种新型ER-alpha单克隆抗体(克隆号DS-1)在乳腺癌分子分型中的应用，证实其与预后相关的ER表达水平高度一致，并优化了免疫组化标准化流程。
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3. **文献名称**：*"ER-alpha Phosphorylation at Serine 118 Modulates Antibody Binding in Hormone-Resistant Cancers"*
**作者**：Murphy LC et al.
**摘要**：探讨ER-alpha磷酸化修饰(如Ser118位点)对常用抗体结合能力的影响，发现某些磷酸化状态可能导致假阴性结果，提示需针对特定表位开发选择性抗体。
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4. **文献名称**：*"Impact of Pre-Analytical Variables on ER-alpha Detection by Immunohistochemistry"*
**作者**：Wolff AC et al.
**摘要**：分析了组织固定时间、抗原修复方法等前处理因素对ER-alpha抗体(克隆SP1)检测结果的影响，提出标准化操作可显著提高诊断准确性。
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如需获取全文，建议通过PubMed或期刊官网检索具体标题及作者。
ER-alpha (estrogen receptor alpha), encoded by the ESR1 gene, is a nuclear hormone receptor that mediates estrogen signaling by binding to estrogen and regulating the transcription of target genes involved in cell proliferation, differentiation, and survival. As a key player in hormone-responsive tissues, ER-alpha is highly expressed in a subset of breast cancers (∼70%) and certain gynecological cancers, making it a critical biomarker for prognosis and therapeutic targeting. ER-alpha antibodies are widely used in immunohistochemistry (IHC) to detect ER-alpha protein expression in tumor tissues, guiding clinical decisions for endocrine therapies such as tamoxifen or aromatase inhibitors.
These antibodies specifically recognize epitopes on the ER-alpha protein, often targeting regions in the N-terminal domain (e.g., clone 1D5) or the ligand-binding domain (e.g., clone 6F11). Their clinical utility relies on standardized IHC protocols and scoring systems (e.g., Allred score) to quantify ER-alpha positivity, which correlates with treatment response. However, variability in antibody sensitivity, tissue fixation methods, and interpretation criteria can affect diagnostic accuracy. Efforts by organizations like ASCO/CAP aim to standardize testing to reduce false negatives/positives. Beyond diagnostics, ER-alpha antibodies are also essential tools in research to study receptor dynamics, post-translational modifications, and interactions with co-regulators in hormone-dependent cancers.
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