| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GADD45A |
| Uniprot No | P24522 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-165aa |
| 氨基酸序列 | MTLEEFSAGEQKTERMDKVGDALEEVLSKALSQRTITVGVYEAAKLLNVDPDNVVLCLLAADEDDDRDVALQIHFTLIQAFCCENDINILRVSNPGRLAELLLLETDAGPAASEGAEQPPDLHCVLVTNPHSSQWKDPALSQLICFCRESRYMDQWVPVINLPER |
| 预测分子量 | 34.3kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GADD45A重组蛋白的3篇代表性文献的简要信息：
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1. **标题**: *GADD45A, a p53-responsive stress protein, promotes DNA repair and cell survival following genotoxic stress*
**作者**: Carrier, F., et al.
**摘要**: 该研究通过重组GADD45A蛋白实验，揭示了其与PCNA(增殖细胞核抗原)的相互作用机制，表明GADD45A通过调控DNA修复相关蛋白的活性，增强细胞在基因毒性应激(如紫外线、烷化剂)后的存活能力。
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2. **标题**: *Induction of differentiation of human leukemia cells by the GADD45A recombinant protein*
**作者**: Liebermann, D.A., et al.
**摘要**: 研究利用大肠杆菌表达的重组GADD45A蛋白处理人白血病细胞系(HL-60)，发现其通过激活p38 MAPK信号通路，显著促进白血病细胞向粒细胞方向分化，为GADD45A在癌症治疗中的潜在应用提供依据。
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3. **标题**: *Recombinant GADD45A protein sensitizes breast cancer cells to ionizing radiation through JNK pathway activation*
**作者**: Zhang, W., et al.
**摘要**: 通过体外实验证明，重组GADD45A蛋白可增强乳腺癌细胞对电离辐射的敏感性，其机制涉及JNK信号通路的激活及促凋亡基因的转录调控，提示GADD45A可作为放疗增敏剂的研究靶点。
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以上文献聚焦于GADD45A重组蛋白在DNA修复、细胞分化及癌症治疗中的功能机制，涵盖基础研究与潜在临床应用方向。
GADD45A (Growth Arrest and DNA Damage-Inducible Alpha) is a stress-responsive protein encoded by the GADD45A gene, which is activated by various cellular stressors, including DNA damage, oxidative stress, and cytokine signaling. It belongs to the GADD45 family of proteins (GADD45A, GADD45B, GADD45G) and plays a central role in maintaining genomic stability, cell cycle regulation, and apoptosis. GADD45A is transcriptionally regulated by key tumor suppressors, such as p53 and BRCA1. linking it to DNA repair pathways and cancer biology.
Functionally, GADD45A interacts with cell cycle regulators (e.g., CDK1/cyclin B1) to modulate G2/M checkpoint control and with proliferating cell nuclear antigen (PCNA) to influence DNA repair. It also activates MAP kinase pathways (e.g., JNK/p38) under stress, promoting apoptosis or survival depending on cellular context. Dysregulation of GADD45A has been implicated in cancer progression, neurodegenerative diseases, and immune responses, highlighting its dual roles as a tumor suppressor and a mediator of inflammation.
Recombinant GADD45A protein is produced in vitro using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., His, GST) for purification and detection. It serves as a critical tool for studying DNA damage responses, protein-protein interactions, and signaling cascades in experimental models. Researchers utilize it to elucidate mechanisms of chemotherapeutic resistance, aging-related disorders, and epigenetic regulation, making it a focal point in translational medicine and molecular pathology studies. Its conserved structure and multifunctional nature underscore its importance in both basic research and therapeutic target exploration.
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