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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GDF2 |
| Uniprot No | Q9UK05 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 300-429aa |
| 氨基酸序列 | HEEDTDGHVAAGSTLARRKRSAGAGSHCQKTSLRVNFEDIGWDSWIIAPKEYEAYECKGGCFFPLADDVTPTKHAIVQTLVHLKFPTKVGKACCVPTKLSPISVLYKDDMGVPTLKYHYEGMSVAECGCR |
| 预测分子量 | 22.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GDF2重组蛋白的3篇代表性文献摘要(文献为虚构示例，供参考)：
1. **文献名称**: *Recombinant GDF2 Promotes Vascular Development via ALK1 Signaling*
**作者**: Zhang et al.
**摘要**: 研究通过哺乳动物细胞系统表达重组人GDF2蛋白，证实其通过激活ALK1/Smad1通路促进内皮细胞分化和血管生成，为治疗缺血性疾病提供潜在策略。
2. **文献名称**: *High-Yield Production of Biologically Active GDF2 in E. coli*
**作者**: Smith et al.
**摘要**: 报道利用大肠杆菌表达系统高效制备重组GDF2.优化纯化工艺后蛋白具有诱导干细胞成骨分化的活性，为大规模生产奠定基础。
3. **文献名称**: *GDF2 Mutants Impair Hepatic Sinusoid Formation in Zebrafish*
**作者**: Wang et al.
**摘要**: 通过重组GDF2蛋白及其突变体功能实验，揭示GDF2在斑马鱼肝窦发育中的关键作用，突变导致血管结构异常，提示与人类肝病相关。
(注：如需真实文献，建议通过PubMed或Sci-Hub检索关键词“recombinant GDF2 protein”获取。)
**Background of GDF2 Recombinant Protein**
Growth Differentiation Factor 2 (GDF2), also known as Bone Morphogenetic Protein 9 (BMP9), is a member of the transforming growth factor-beta (TGF-β) superfamily. It plays critical roles in regulating cellular processes such as differentiation, proliferation, and angiogenesis. GDF2 is primarily synthesized as a precursor protein, which undergoes proteolytic cleavage to release the mature, biologically active dimer. This secreted glycoprotein signals through a receptor complex involving type I (ALK1) and type II (BMPR2) serine/threonine kinase receptors, activating downstream SMAD signaling pathways.
GDF2 is notably expressed in the liver and contributes to vascular development and homeostasis. It regulates endothelial cell function, promoting vessel stability and quiescence, and is implicated in metabolic processes, including iron homeostasis and glucose metabolism. Its role in maintaining vascular integrity has drawn attention in research on cardiovascular diseases, hereditary hemorrhagic telangiectasia (HHT), and pulmonary arterial hypertension (PAH).
Recombinant GDF2 is produced using biotechnological platforms, such as mammalian expression systems (e.g., HEK293 or CHO cells), to ensure proper post-translational modifications and bioactivity. Purification processes typically involve chromatography techniques to achieve high purity and specificity.
In therapeutic contexts, GDF2 has potential applications in regenerative medicine, such as promoting tissue repair, angiogenesis in ischemic conditions, or enhancing stem cell differentiation. However, challenges remain, including optimizing its stability, delivery mechanisms, and understanding context-dependent signaling effects. Ongoing studies aim to harness its dual role in vascular biology and metabolism while mitigating risks of off-target effects.
Overall, GDF2 recombinant protein represents a promising yet complex tool for both basic research and clinical development in vascular and metabolic disorders.
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