| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ORF26 |
| Entrez GeneID | 4961509 |
| clone | 2F6B8 |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of KSHV ORF26 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及KSHV ORF26抗体的参考文献及其摘要要点：
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1. **文献名称**：*Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma*
**作者**：Chang Y, et al.
**摘要**：该研究首次发现KSHV(HHV-8)与卡波西肉瘤的关联，通过PCR扩增ORF26基因片段确认病毒存在，并提及利用ORF26抗体进行病毒颗粒的免疫组化检测，支持病毒在肿瘤组织中的表达。
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2. **文献名称**：*Development of a novel monoclonal antibody specific for latent nuclear antigen of KSHV/HHV-8 and its application in immunohistochemical diagnosis*
**作者**：Katano H, et al.
**摘要**：研究团队制备了针对KSHV ORF26编码蛋白的单克隆抗体，验证其在免疫组化(IHC)和免疫印迹(Western blot)中的特异性，成功用于检测临床样本中的病毒潜伏感染。
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3. **文献名称**：*Distribution of human herpesvirus-8 latently infected cells in Kaposi's sarcoma, multicentric Castleman's disease, and primary effusion lymphoma*
**作者**：Dupin N, et al.
**摘要**：利用ORF26抗体结合原位杂交技术，分析了KSHV在不同疾病(如卡波西肉瘤)中的分布模式，证明ORF26抗体在定位病毒潜伏感染细胞中的有效性。
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**备注**：若需具体实验方法或抗体货号，可进一步查阅上述文献的“Materials and Methods”部分或补充材料。
The KSHV ORF26 antibody is a key reagent used in the study of Kaposi's sarcoma-associated herpesvirus (KSHV), also known as human herpesvirus 8 (HHV-8). KSHV is a gammaherpesvirus linked to Kaposi's sarcoma, primary effusion lymphoma, and multicentric Castleman's disease. ORF26 encodes a conserved structural protein component of the viral capsid, critical for virion assembly and maturation. Antibodies targeting ORF26 are primarily employed to detect KSHV lytic replication in infected cells or tissues.
These antibodies are widely utilized in techniques like immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting to identify KSHV-infected cells, particularly during the lytic phase of the viral life cycle. ORF26 is transcribed late in lytic replication, making its detection a marker of active viral proliferation. Researchers often use ORF26 antibodies in combination with latency-associated antigens (e.g., LANA) to distinguish between latent and lytic infection stages.
The ORF26 protein contains conserved epitopes across KSHV strains, ensuring broad reactivity in clinical and experimental samples. Its detection is valuable in both basic virology (e.g., studying viral replication mechanisms) and clinical diagnostics (e.g., confirming KSHV presence in tumor biopsies). Commercial ORF26 antibodies are typically raised against peptide sequences derived from the N-terminal or central regions of the protein. Validation includes testing on KSHV-positive cell lines (e.g., BCBL-1 or TPA-induced BC-3) and comparison with PCR-based viral load assays. Proper controls are essential due to potential cross-reactivity with other herpesvirus homologs or nonspecific binding in tissue samples.
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