| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXO3 |
| Uniprot No | Q9UK99 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-471aa |
| 氨基酸序列 | MAAMETETAPLTLESLPTDPLLLILSFLDYRDLINCCYVSRRLSQLSSHDPLWRRHCKKYWLISEEEKTQKNQCWKSLFIDTYSDVGRYIDHYAAIKKAWDDLKKYLEPRCPRMVLSLKEGAREEDLDAVEAQIGCKLPDDYRCSYRIHNGQKLVVPGLLGSMALSNHYRSEDLLDVDTAAGGFQQRQGLKYCLPLTFCIHTGLSQYIAVEAAEGRNKNEVFYQCPDQMARNPAAIDMFIIGATFTDWFTSYVKNVVSGGFPIIRDQIFRYVHDPECVATTGDITVSVSTSFLPELSSVHPPHYFFTYRIRIEMSKDALPEKACQLDSRYWRITNAKGDVEEVQGPGVVGEFPIISPGRVYEYTSCTTFSTTSGYMEGYYTFHFLYFKDKIFNVAIPRFHMACPTFRVSIARLEMGPDEYEEMEEEEEEEEEEDEDDDSADMDESDEDDEEERRRRVFDVPIRRRRCSRLF |
| 预测分子量 | 56.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FBXO3重组蛋白的3篇参考文献示例(注：文献信息为模拟生成，仅供参考)：
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1. **标题**: *FBXO3 promotes NF-κB signaling by mediating degradation of the deubiquitinase CYLD*
**作者**: Chen BB et al.
**摘要**: 本研究通过重组FBXO3蛋白体外泛素化实验，证实FBXO3作为SCF复合体的适配蛋白，靶向去泛素化酶CYLD进行降解，从而激活NF-κB通路并加剧炎症反应。
2. **标题**: *Structural basis of FBXO3 substrate recognition in ubiquitination pathways*
**作者**: Li Y et al.
**摘要**: 利用重组表达的人源FBXO3蛋白进行X射线晶体学分析，揭示了其F-box结构域与Skp1的结合界面，以及C端区域识别特定底物基序的分子机制。
3. **标题**: *FBXO3-mediated degradation of DAB2IP promotes tumor metastasis*
**作者**: Wang X et al.
**摘要**: 研究通过体外重组FBXO3蛋白与DAB2IP的互作实验，发现FBXO3通过泛素化降解肿瘤抑制因子DAB2IP，促进EMT过程及癌症转移，为靶向干预提供新策略。
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**备注**：实际文献需通过PubMed/Google Scholar等平台检索关键词“FBXO3 recombinant”、“FBXO3 ubiquitin”等获取，建议结合具体研究方向筛选近年高影响力论文。
FBXO3 is a member of the F-box protein family, which serves as a critical component of the Skp1-Cullin-F-box (SCF) E3 ubiquitin ligase complex. This complex plays a central role in the ubiquitin-proteasome system, a major pathway for targeted protein degradation in eukaryotic cells. As an F-box protein, FBXO3 acts as a substrate-recognition subunit, determining the specificity of the SCF complex by binding to phosphorylated or otherwise modified target proteins. This interaction facilitates their ubiquitination and subsequent proteasomal degradation, thereby regulating key cellular processes such as cell cycle progression, signal transduction, and stress responses.
Recombinant FBXO3 protein is engineered in vitro using expression systems like *E. coli* or mammalian cell cultures, often fused with affinity tags (e.g., His-tag, GST-tag) to enable purification and detection. Its production allows researchers to study FBXO3's structural and functional properties, including its interactions with binding partners (e.g., Skp1. substrates), post-translational modifications, and regulatory mechanisms. Notably, FBXO3 has been implicated in inflammatory diseases and cancer due to its role in degrading tumor suppressors or stabilizing pro-inflammatory factors. For example, it promotes NF-κB signaling by mediating the ubiquitination of the inhibitor PIAS1. thereby enhancing cytokine production. Dysregulation of FBXO3 is linked to pathological conditions, making it a potential therapeutic target.
The availability of recombinant FBXO3 supports drug discovery efforts, particularly in screening small-molecule inhibitors to modulate its activity. Its study also advances understanding of ubiquitination dynamics in cellular homeostasis and disease pathogenesis.
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