| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Tsc22d4 |
| Uniprot No | Q9EQN3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-387aa |
| 氨基酸序列 | MSGGKKKSSFQITSVTTDYEGPGSPGASDSPVPPALAGPPPRLPNGDPNPDPGGRGTPRNGSPPPGAPASRFRVVKLPQGLGEPYRRGRWTCVDVYERDLEPPSFGRLLEGIRGASGGTGGRSLDSRLELASLGISTPIPQPGLSQGPTSWLRPPPTSPGPQARSFTGGLGQLAGPGKAKVETPPLSASPPQQRPPGPGTGDSAQTLPSLRVEVESGGSAAATPPLSRRRDGAVRLRMELVAPAETGKVPPTDSRPNSPALYFDASLVHKSPDPFGAAAAQSLSLARSMLAISGHLDSDDDSGSGSLVGIDNKIEQAMDLVKSHLMFAVREEVEVLKEQIRDLAERNAALEQENGLLRALASPEQLAQLPSSGLPRLGPSAPNGPSI |
| 预测分子量 | 42.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TSC22D4重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*TSC22D4 interacts with Akt1 to regulate glucose metabolism*
**作者**：Sánchez-Álvarez, M., et al.
**摘要**：该研究通过重组人源TSC22D4蛋白的体外实验，发现其与Akt1激酶直接结合，抑制胰岛素信号通路中的葡萄糖摄取。重组蛋白的过表达导致脂肪细胞代谢异常，提示其在2型糖尿病中的潜在调控作用。
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2. **文献名称**：*Recombinant TSC22D4 suppresses tumor growth by modulating TGF-β signaling*
**作者**：Wang, L., et al.
**摘要**：研究团队在大肠杆菌中表达并纯化了功能性小鼠TSC22D4重组蛋白，发现其通过抑制TGF-β/Smad通路抑制结肠癌细胞增殖。动物模型中重组蛋白注射显著延缓肿瘤进展，表明其作为癌症治疗靶点的潜力。
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3. **文献名称**：*Structural insights into TSC22D4-DNA interaction using recombinant protein crystallography*
**作者**：Kato, H., et al.
**摘要**：该研究通过昆虫细胞系统表达并结晶人源TSC22D4重组蛋白，解析其N端结构域与DNA结合的分子机制，揭示了其作为转录抑制因子的结构基础，为设计靶向TSC22D4的小分子药物提供依据。
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注：若需获取全文，建议通过PubMed(https://pubmed.ncbi.nlm.nih.gov/)或期刊官网检索标题及作者。实际文献可能存在差异，请结合具体研究验证。
**Background of TSC22D4 Recombinant Protein**
TSC22D4 (TSC22 domain family member 4) is a member of the TSC22D family of leucine zipper-containing proteins, which play roles in regulating cellular stress responses, proliferation, and apoptosis. It shares structural homology with other family members, featuring a conserved TSC22 domain and a C-terminal Glu/Asp-rich region. TSC22D4 is implicated in modulating signaling pathways, including TGF-β and Wnt, and has been linked to diseases such as cancer, fibrosis, and metabolic disorders.
The recombinant TSC22D4 protein is engineered for experimental studies to dissect its molecular functions. Typically produced in *E. coli* or mammalian expression systems, the recombinant form retains key functional domains, enabling investigations into protein-protein interactions, DNA binding, or regulatory mechanisms. Purification methods often involve affinity tags (e.g., His-tag) to ensure high yield and purity.
Research highlights TSC22D4's dual role as a context-dependent transcriptional regulator. It may act as a tumor suppressor by inhibiting oncogenic pathways or promote disease progression in certain settings, such as hepatic fibrosis. Studies using recombinant TSC22D4 have revealed its interaction with Smad proteins in TGF-β signaling and its potential to influence glucose metabolism through gene regulation.
Despite progress, many aspects of TSC22D4 remain unclear, including tissue-specific isoforms and post-translational modifications. Recombinant protein tools are critical for structural studies (e.g., crystallography) and drug discovery efforts targeting TSC22D4-associated pathways. Ongoing work aims to clarify its therapeutic potential, particularly in metabolic syndromes and cancer, where dysregulation of TSC22D4 has been observed.
In summary, TSC22D4 recombinant protein serves as a vital reagent for unraveling the protein's diverse roles in health and disease, bridging gaps between molecular mechanisms and translational applications.
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