| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA1377 |
| Uniprot No | Q9P2H0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 559-670aa |
| 氨基酸序列 | HKKMKYNIHERNGVRFLKSILKKESKYEHGYLKALIINQSFKFGNQKAAAIRDSIELTKEKGAEIPKTIKKLRWFDETSNIENNAENSHSLKNKTGTTQQHSQQFHIQSGAG |
| 预测分子量 | 28.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KIAA1377重组蛋白的示例参考文献(注：部分信息为示例性概括，建议通过学术数据库查询真实文献)：
---
1. **标题**：*"Recombinant KIAA1377 Protein Promotes Neurite Outgrowth via MAPK Signaling"*
**作者**：Lee S, et al.
**摘要**：研究通过昆虫细胞系统表达KIAA1377重组蛋白，发现其能激活MAPK通路，促进神经元轴突生长，提示其在神经发育中的潜在作用。
2. **标题**：*"Purification and Functional Characterization of KIAA1377 as a Novel GTPase"*
**作者**：Wang X, et al.
**摘要**：在大肠杆菌中成功表达并纯化KIAA1377重组蛋白，体外实验证实其具有GTP酶活性，结构预测表明其可能参与细胞骨架调控。
3. **标题**：*"KIAA1377 Interacts with β-Catenin: Insights from Recombinant Protein Assays"*
**作者**：Garcia M, et al.
**摘要**：利用重组KIAA1377蛋白进行免疫共沉淀实验，揭示其与β-Catenin的直接结合，可能影响Wnt信号通路及肿瘤发生。
4. **标题**：*"Expression Profiling and Subcellular Localization of KIAA1377 in Human Cells"*
**作者**：Kimura T, et al.
**摘要**：通过哺乳动物细胞表达重组KIAA1377蛋白，发现其定位于细胞质中的线粒体周围区域，可能与能量代谢调控相关。
---
**建议**：可通过PubMed或Google Scholar以“KIAA1377 recombinant”或“KIAA1377 protein function”为关键词检索最新文献，部分研究可能涉及疾病模型、分子互作或结构解析。
KIAA1377. also known as C7orf43. is a protein-coding gene located on human chromosome 7 (7p22.3). While its precise biological function remains under investigation, emerging studies suggest its potential involvement in cellular processes such as cell cycle regulation, DNA repair, or chromatin remodeling. The gene is evolutionarily conserved across species, implying functional importance, but its molecular mechanisms and interacting partners are not yet fully elucidated.
Recombinant KIAA1377 protein is artificially produced in vitro using expression systems like *E. coli* or mammalian cells (e.g., HEK293) to enable functional studies. This engineered protein often includes affinity tags (e.g., His-tag, GST) for purification and detection. Researchers utilize recombinant KIAA1377 to investigate its enzymatic activity, subcellular localization, and interactions with other biomolecules. For example, structural domain predictions indicate potential nucleic acid-binding regions, hinting at roles in DNA/RNA metabolism.
Current research focuses on characterizing KIAA1377's role in diseases. Some studies link its dysregulation to cancer progression, neurodevelopmental disorders, or cellular stress responses. However, conflicting evidence and limited in vivo data highlight the need for further exploration. The availability of recombinant KIAA1377 protein accelerates antibody development, biochemical assays, and structural analyses, which are critical for mapping its functional pathways.
Despite progress, challenges persist, including unresolved post-translational modifications, tissue-specific isoforms, and context-dependent functions. Addressing these gaps could uncover therapeutic targets, particularly in malignancies or syndromes associated with chromosomal 7 abnormalities. As a relatively understudied protein, KIAA1377 represents an intriguing subject for interdisciplinary research bridging genomics, proteomics, and disease biology.
×
