| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPAN |
| Uniprot No | Q9NQ55 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-473aa |
| 氨基酸序列 | MGQSGRSRHQKRARAQAQLRNLEAYAANPHSFVFTRGCTGRNIRQLSLDVRRVMEPLTASRLQVRKKNSLKDCVAVAGPLGVTHFLILSKTETNVYFKLMRLPGGPTLTFQVKKYSLVRDVVSSLRRHRMHEQQFAHPPLLVLNSFGPHGMHVKLMATMFQNLFPSINVHKVNLNTIKRCLLIDYNPDSQELDFRHYSIKVVPVGASRGMKKLLQEKFPNMSRLQDISELLATGAGLSESEAEPDGDHNITELPQAVAGRGNMRAQQSAVRLTEIGPRMTLQLIKVQEGVGEGKVMFHSFVSKTEEELQAILEAKEKKLRLKAQRQAQQAQNVQRKQEQREAHRKKSLEGMKKARVGGSDEEASGIPSRTASLELGEDDDEQEDDDIEYFCQAVGEAPSEDLFPEAKQKRLAKSPGRKRKRWEMDRGRGRLCDQKFPKTKDKSQGAQARRGPRGASRDGGRGRGRGRPGKRVA |
| 预测分子量 | 55.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPAN重组蛋白的3篇参考文献示例(注：文献为虚构示例，仅用于演示格式)：
1. **文献名称**: "PPAN重组蛋白的克隆表达及其在核仁功能研究中的应用"
**作者**: Zhang L. et al.
**摘要**: 本研究成功构建了PPAN基因的重组表达载体，在大肠杆菌中高效表达并纯化PPAN蛋白。通过体外实验证实，重组PPAN参与调控核糖体RNA加工，对维持核仁结构完整性具有关键作用。
2. **文献名称**: "Structural insights into PPAN protein complex by cryo-EM"
**作者**: Müller R. et al.
**摘要**: 利用冷冻电镜技术解析了重组PPAN蛋白与其结合伴侣的复合物三维结构，揭示了PPAN通过特定的结构域介导蛋白质互作，为理解其在干细胞自我更新中的分子机制提供结构基础。
3. **文献名称**: "PPAN knockdown induces apoptosis via mitochondrial pathway: Evidence from recombinant protein rescue experiments"
**作者**: Kim S. et al.
**摘要**: 通过RNA干扰敲低细胞中内源性PPAN表达导致线粒体依赖性凋亡，而外源添加重组PPAN蛋白可显著逆转该表型，证明PPAN通过调控Bcl-2家族蛋白发挥抗凋亡功能。
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**说明**：实际文献检索建议通过PubMed/Google Scholar以"PPAN recombinant protein"、"PPAN cloning"等关键词查找，重点关注其分子功能、疾病关联或生产方法的研究。若需真实文献，请提供更具体的背景信息。
**Background of PPAN Recombinant Protein**
PPAN (Pervasive Proliferation-Associated Nuclear protein) is a nuclear protein encoded by the *PPAN* gene, implicated in diverse cellular processes, including ribosome biogenesis, cell proliferation, and apoptosis. It is evolutionarily conserved and shares homology with the yeast protein SSF1. which regulates RNA metabolism and stress responses. Structurally, PPAN contains a conserved Ssf1p-like domain, facilitating interactions with RNA and proteins, and plays a role in nucleolar organization, a critical site for ribosomal RNA (rRNA) synthesis and processing.
Studies highlight PPAN's dual role in cell survival and death. Under normal conditions, PPAN supports ribosome assembly and cell proliferation by stabilizing rRNA precursors. However, under stress (e.g., DNA damage or nutrient deprivation), PPAN translocates to the nucleoplasm, where it interacts with pro-apoptotic factors like p53. promoting cell cycle arrest or apoptosis. This balance positions PPAN as a potential regulator of tumorigenesis, with dysregulation linked to cancers and neurodegenerative disorders.
Recombinant PPAN proteins are engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its molecular mechanisms. These proteins retain functional domains, enabling in vitro analyses of PPAN-RNA/DNA interactions, enzymatic activity, or binding partners. Recombinant PPAN is pivotal for structural studies (e.g., crystallography), functional assays (e.g., ribosome assembly models), and drug screening targeting PPAN-related pathways.
Overall, PPAN recombinant proteins serve as essential tools for unraveling its biological roles and exploring therapeutic strategies in diseases marked by aberrant cell proliferation or apoptosis.
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