| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Fam83d |
| Uniprot No | Q9D7I8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-585aa |
| 氨基酸序列 | MAARFELLDDLPAACLSPCGPPNPTELFSEARRLALEQLLAGGPDAWAAFLRRERLGRFLNADEVREVLGAAERPGEDGAAVAEDSFGSSHECSSGTYFPEQSDLEPPALELGWPSFYQGAYRGATRVEAHFQPRGAGAGGPYGCKDALRQQLRSAREVIAVVMDVFSDIDIFRDLQESCRKRGVAVYILLDQTLLPHFLDMCMDLRVHPEQEKLMTVRTITGNIYYARSGTKVVGKVHEKFTLIDGIRVATGSYSFTWTDGKLNSSNLVILSGQVVEHFDLEFRILYAQSEPISSKLLSNFQINSKFDHLADRKPQSKEPTLGNLLRMRLARLSSTPRKSNLGPEEPPKDRAKPKRPDSEASTISDEDYFHSHKDQLEDSKVADAATQTEPREEMAAVSLSEVGTQTSSSMMCVGTQTTVVTRAASSQATVWSKSTTTQTEADESFLPQGAQSKEGSPASKMSVSRSSSVRSSSSVSSQGSLASSVSSHVSLTAADLHTPAYPKYLGLGTPHLDLCLRDSFRNLSKERQVHFTGIRSRLTQMLTVLSRRTLFTEHYLSYSPGSFTRASTNLVSVRDIALYPPYQ |
| 预测分子量 | 66.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Fam83d重组蛋白的3篇参考文献及其摘要概括：
1. **《FAM83D promotes cell proliferation and motility by downregulating tumor suppressor gene FBXW7》**
- 作者：Zhang Y, et al.
- 摘要：研究发现FAM83D通过抑制泛素连接酶FBXW7.稳定癌蛋白c-Myc和Cyclin E1.促进肝癌细胞增殖和迁移，并与患者不良预后相关。
2. **《FAM83D binds to CK1α and regulates Wnt/β-catenin signaling in colorectal cancer》**
- 作者：Wang H, et al.
- 摘要：揭示FAM83D与酪蛋白激酶CK1α相互作用，抑制β-catenin降解，激活Wnt通路，促进结直肠癌转移，可能成为潜在治疗靶点。
3. **《FAM83D interacts with MAPK signaling to drive breast cancer progression》**
- 作者：Guo J, et al.
- 摘要：在乳腺癌中，FAM83D通过增强MAPK通路活性，促进细胞增殖和化疗耐药，敲低FAM83D可抑制肿瘤生长并提高化疗敏感性。
(注：以上文献信息为示例，实际研究需根据具体论文调整。)
**Background of FAM83D Recombinant Protein**
FAM83D (Family with sequence similarity 83 member D) is a member of the FAM83 protein family, which is conserved across eukaryotes and implicated in diverse cellular processes, including cell proliferation, differentiation, and signaling. FAM83D, also known as CHICA, is a 58 kDa protein encoded by the *FAM83D* gene located on human chromosome 20q11.21. It contains a conserved N-terminal domain unique to the FAM83 family and a putative nuclear localization signal.
Functionally, FAM83D plays a critical role in mitotic regulation. It interacts with protein kinases, such as Polo-like kinase 1 (PLK1), and is essential for proper spindle assembly, chromosome segregation, and cell cycle progression. Studies suggest FAM83D regulates the localization and activity of PLK1 during mitosis, ensuring accurate division. Dysregulation of FAM83D is linked to genomic instability, a hallmark of cancer. Overexpression of FAM83D has been observed in various cancers (e.g., breast, liver, lung) and correlates with poor prognosis, tumor proliferation, and metastasis.
Recombinant FAM83D protein is engineered for research applications, typically produced in bacterial or mammalian expression systems. It retains functional domains for studying protein-protein interactions, kinase activity, and mitotic mechanisms in vitro. Researchers utilize it to explore FAM83D's oncogenic potential, screen inhibitors, or validate antibodies. Challenges include optimizing solubility and post-translational modifications (e.g., phosphorylation) critical for its activity.
Despite progress, FAM83D's precise molecular mechanisms and therapeutic potential remain under investigation, highlighting its significance in both basic biology and translational oncology.
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