| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BANK1 |
| Uniprot No | Q8NDB2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-785aa |
| 氨基酸序列 | MLPAAPGKGLGSPDPAPCGPAPPGNTKDIIMIYEEDAEEWALYLTEVFLHVVKREAILLYRLENFSFRHLELLNLTSYKCKLLILSNSLLRDLTPKKCQFLEKILHSPKSVVTLLCGVKSSDQLYELLNISQSRWEISTEQEPEDYISVIQSIIFKDSEDYFEVNIPTDLRAKHSGEISERKEIEELSEASRNTIPLAVVLPTEIPCENPGEIFIILRDEVIGDTVEVEFTSSNKRIRTRPALWNKKVWCMKALEFPAGSVHVNVYCDGIVKATTKIKYYPTAKAKECLFRMADSGESLCQNSIEELDGVLTSIFKHEIPYYEFQSLQTEICSQNKYTHFKELPTLLHCAAKFGLKNLAIHLLQCSGATWASKMKNMEGSDPAHIAERHGHKELKKIFEDFSIQEIDINNEQENDYEEDIASFSTYIPSTQNPAFHHESRKTYGQSADGAEANEMEGEGKQNGSGMETKHSPLEVGSESSEDQYDDLYVFIPGADPENNSQEPLMSSRPPLPPPRPVANAFQLERPHFTLPGTMVEGQMERSQNWGHPGVRQETGDEPKGEKEKKEEEKEQEEEEDPYTFAEIDDSEYDMILANLSIKKKTGSRSFIINRPPAPTPRPTSIPPKEETTPYIAQVFQQKTARRQSDDDKFCGLPKKQDRARIESPAFSTLRGCLTDGQEELILLQEKVKNGKMSMDEALEKFKHWQMGKSGLEMIQQEKLRQLRDCIIGKRPEEENVYNKLTIVHHPGGKETAHNENKFYNVHFSNKLPARPQVEKEFGFCCKKDH |
| 预测分子量 | 91.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BANK1重组蛋白的3篇参考文献示例(注：文献信息为模拟生成，仅供参考)：
1. **文献名称**：*Structural and functional analysis of BANK1 in B-cell signaling*
**作者**：Smith A, et al.
**摘要**：通过重组BANK1蛋白表达及晶体结构解析，揭示了其ANK结构域与IP3R的相互作用机制，阐明其在B细胞受体信号传导中的支架作用。
2. **文献名称**：*BANK1 polymorphisms and lupus pathogenesis: Recombinant protein-based interaction studies*
**作者**：Chen L, et al.
**摘要**：利用重组BANK1蛋白变体进行体外结合实验，证明特定基因突变(如R61H)影响其与TLR9的结合能力，可能与系统性红斑狼疮的易感性相关。
3. **文献名称**：*Recombinant BANK1 as a tool for autoimmune disease biomarker discovery*
**作者**：Yamamoto K, et al.
**摘要**：开发了基于重组BANK1蛋白的ELISA检测方法，发现血清中抗BANK1抗体水平与类风湿关节炎活动度显著相关，提示其作为生物标志物的潜力。
*提示*：实际文献需通过PubMed/Google Scholar检索关键词“BANK1 recombinant protein”或结合具体研究领域筛选。部分真实文献可能涉及：
- BANK1磷酸化调控机制
- 与BCR/TLR通路分子的互作验证
- 疾病相关突变体的功能丧失研究
**Background of BANK1 Recombinant Protein**
BANK1 (B-cell scaffold protein with ankyrin repeats 1) is a cytoplasmic scaffold protein predominantly expressed in B lymphocytes. It plays a regulatory role in B-cell receptor (BCR)-mediated signaling by coordinating interactions between signaling molecules. Structurally, BANK1 contains an N-terminal ankyrin repeat domain, a coiled-coil region, and a C-terminal region with multiple tyrosine phosphorylation sites. These domains enable BANK1 to act as an adaptor, facilitating the assembly of signaling complexes.
Genetic studies have linked *BANK1* polymorphisms to autoimmune diseases, particularly systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Dysregulated BANK1 expression or function may contribute to aberrant B-cell activation and autoantibody production, highlighting its therapeutic relevance.
Recombinant BANK1 protein is produced in vitro using expression systems (e.g., *E. coli*, mammalian cells) to study its biochemical properties, interactions, and role in immune pathways. Purified BANK1 recombinant proteins retain functional domains, enabling researchers to investigate phosphorylation events (e.g., Tyr823), binding partners (e.g., Lyn kinase, IP3 receptors), and downstream effects on calcium signaling and B-cell activation.
Applications include structural analysis, protein-protein interaction assays, and screening for therapeutic modulators. BANK1 recombinant proteins also aid in developing disease models to dissect its contribution to autoimmunity, offering insights for targeted drug development. Research on BANK1 continues to advance understanding of B-cell biology and immune dysregulation in autoimmune disorders.
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