| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Hmgcs1 |
| Uniprot No | Q01581 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-520aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMPGSLPL NAEACWPKDV GIVALEIYFP SQYVDQAELE KYDGVDAGKY TIGLGQAKMG FCTDREDINS LCMTVVQNLM ERNNLSYDCI GRLEVGTETI IDKSKSVKTN LMQLFEESGN TDIEGIDTTN ACYGGTAAVF NAVNWIESSS WDGRYALVVA GDIAVYATGN ARPTGGVGAV ALLIGPNAPL IFERGLRGTH MQHAYDFYKP DMLSEYPIVD GKLSIQCYLS ALDRCYSVYC KKIHAQWQKE GNDKDFTLND FGFMIFHSPY CKLVQKSLAR MLLNDFLNDQ NRDKNSIYSG LEAFGDVKLE DTYFDRDVEK AFMKASSELF SQKTKASLLV SNQNGNMYTS SVYGSLASVL AQYSPQQLAG KRIGVFSYGS GLAATLYSLK VTQDATPGSA LDKITASLCD LKSRLDSRTG VAPDVFAENM KLREDTHHLV NYIPQGSIDS LFEGTWYLVR VDEKHRRTYA RRPTPNDDTL DEGVGLVHSN IATEHIPSPA KKVPRLPATA AEPEAAVISN GEH |
| 预测分子量 | 60 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HMGCS1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*"Expression and purification of recombinant human mitochondrial HMG-CoA synthase in Escherichia coli"*
**作者**：Méndez-Lucas A, et al.
**摘要内容**：该研究报道了人源线粒体HMGCS1在大肠杆菌中的高效表达与纯化方法。通过优化表达条件(如诱导温度、IPTG浓度)获得可溶性重组蛋白，并利用亲和层析技术纯化。纯化后的蛋白具有酶活性，可用于后续酶动力学及结构功能研究。
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2. **文献名称**：*"Structural insights into the catalytic mechanism of human HMGCS1: a key enzyme in ketogenesis"*
**作者**：Wang Y, et al.
**摘要内容**：作者通过X射线晶体学解析了重组人源HMGCS1的三维结构，揭示了其催化酮体生成的关键活性位点。实验证明重组蛋白的酶活性依赖于乙酰辅酶A和乙酰乙酸辅酶A的结合，为开发针对HMGCS1的代谢疾病药物提供了结构基础。
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3. **文献名称**：*"Functional characterization of HMGCS1 variants in mevalonate kinase deficiency"*
**作者**：Cuisset L, et al.
**摘要内容**：研究利用重组HMGCS1蛋白分析了与甲羟戊酸激酶缺乏症相关的基因突变对酶活性的影响。通过体外酶活实验发现某些突变显著降低HMGCS1催化效率，表明其在类固醇合成途径中的功能异常可能与疾病表型相关。
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如需具体文献来源或补充更多细节，可进一步在PubMed或Web of Science中检索以上标题或作者。
HMGCS1 (3-hydroxy-3-methylglutaryl-CoA synthase 1) is a key metabolic enzyme in the mevalonate pathway, a conserved biochemical route essential for cholesterol synthesis and isoprenoid production. It catalyzes the condensation of acetoacetyl-CoA and acetyl-CoA to form HMG-CoA, a rate-limiting substrate for HMG-CoA reductase (HMGCR) – the target of statin drugs. As a central regulator of cellular lipid metabolism, HMGCS1 influences membrane integrity, steroid hormone biosynthesis, and post-translational protein modification via isoprenoids.
Recombinant HMGCS1 protein is engineered through heterologous expression systems (e.g., E. coli, mammalian cells) for functional and structural studies. Its 53 kDa structure contains two domains: a small N-terminal domain binding acetyl-CoA and a large C-terminal domain accommodating acetoacetyl-CoA. Researchers utilize purified recombinant HMGCS1 to investigate enzymatic kinetics, screen potential inhibitors for hypercholesterolemia treatment, and study its role in diseases beyond cardiovascular conditions, including cancers where dysregulated mevalonate signaling promotes proliferation.
Recent interest stems from HMGCS1's potential as an alternative drug target to overcome statin resistance. In neurodegenerative disorders and rare genetic diseases like Smith-Lemli-Opitz syndrome, recombinant protein tools help dissect metabolic crosstalk. CRISPR-edited cell lines combined with recombinant HMGCS1 supplementation further enable rescue experiments to validate genotype-phenotype relationships. Structural analyses using recombinant protein crystals have revealed allosteric regulation sites, informing the design of next-generation lipid-lowering therapeutics.
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