| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Abhd11 |
| Uniprot No | Q8NFV4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-315aa |
| 氨基酸序列 | MRAGQQLASMLRWTRAWRLPREGLGPHGPSFARVPVAPSSSSGGRGGAEPRPLPLSYRLLDGEAALPAVVFLHGLFGSKTNFNSIAKILAQQTGRRVLTVDARNHGDSPHSPDMSYEIMSQDLQDLLPQLGLVPCVVVGHSMGGKTAMLLALQRPELVERLIAVDISPVESTGVSHFATYVAAMRAINIADELPRSRARKLADEQLSSVIQDMAVRQHLLTNLVEVDGRFVWRVNLDALTQHLDKILAFPQRQESYLGPTLFLLGGNSQFVHPSHHPEIMRLFPRAQMQTVPNAGHWIHADRPQDFIAAIRGFLV |
| 预测分子量 | 41.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Abhd11重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: "ABHD11 maintains 2-arachidonoylglycerol biosynthesis in macrophages through metabolic integration of proteolytic and biosynthetic pathways"
**作者**: Lee J, et al.
**摘要**: 该研究通过重组ABHD11蛋白实验，揭示了其在巨噬细胞中调控2-AG生物合成的关键作用，发现ABHD11通过整合溶酶体蛋白酶活性和脂质代谢通路维持内源性大麻素稳态。
2. **文献名称**: "Structural and functional characterization of the ABHD11 protein: A potential role in mitochondrial dysfunction"
**作者**: Smith R, et al.
**摘要**: 利用重组ABHD11蛋白进行结构解析和酶活分析，发现其具有水解酶活性，并可能通过调控线粒体膜脂代谢参与神经退行性疾病的发生机制。
3. **文献名称**: "ABHD11 modulates ovarian cancer progression via the TGF-β signaling pathway"
**作者**: Zhang Y, et al.
**摘要**: 研究通过体外重组ABHD11蛋白的功能实验，证明其通过抑制TGF-β/Smad信号通路抑制卵巢癌细胞迁移，提示其作为肿瘤抑制因子的潜在治疗价值。
注：上述文献信息为模拟示例，实际研究中请通过PubMed/Google Scholar以关键词“ABHD11 recombinant”检索最新文献。若研究较少，可扩展至该蛋白家族(如ABHD酶类)或相关通路的研究。
ABHD11 (α/β-hydrolase domain-containing protein 11) is a member of the ABHD enzyme family, characterized by a conserved α/β-hydrolase fold that supports diverse enzymatic activities. This protein, encoded by the ABHD11 gene in humans, is evolutionarily conserved across eukaryotes, suggesting fundamental biological roles. Structurally, it contains a catalytic triad (serine-histidine-aspartate) typical of hydrolytic enzymes, though its precise substrate specificity remains under investigation. ABHD11 is implicated in lipid metabolism and cellular signaling pathways, with potential roles in mitochondrial function and membrane dynamics.
Recombinant ABHD11 protein is engineered for experimental studies to overcome challenges in isolating native forms. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), it often includes affinity tags (e.g., His-tag) for purification. Its recombinant form enables biochemical characterization, including enzymatic assays to identify substrates and inhibitors, aiding drug discovery. ABHD11 has drawn attention due to its association with chromosomal microdeletion disorders (e.g., 17p13.3 deletion syndrome) linked to neurodevelopmental defects. Studies suggest it interacts with proteins like LIS1. a regulator of neuronal migration, hinting at roles in brain development.
Despite progress, ABHD11's full physiological functions remain elusive. Research leverages recombinant protein to explore its involvement in diseases, including cancer and metabolic disorders, where altered lipid processing is implicated. Ongoing work aims to clarify its enzymatic mechanisms and therapeutic potential, positioning ABHD11 as a target for precision medicine strategies.
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