| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Arid5a |
| Uniprot No | Q3U108 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-590aa |
| 氨基酸序列 | MAAPPAKGNTEQSEEGDLPQLPVSPKPDDEQSRSQSPTQLQDSPEAGGEQEEEQAFLVSLYKFMKERHTPIERVPHLGFKQINLWKIYKAVEKLGAYELVTGRRLWKNVYDELGGSPGSTSAATCTRRHYERLVLPYVRHLKGEDDKPLPPTKPRKQYKMAKELRGDDGTTEKLKKAKDSEERRVEQTTPGKTKSDATGQTQLPCQGSSRDSTEQLGPVSGPSPPLTGASSCPEAYKRLLSSFYCKGAHGIMSPLAKKKLLAQVSKAEALQCQEEGCRHGARSPNKDIQDSPQNLRGPAENSEHQLTPREGLQAPGGSTRMEAQVGPCPTAPMFSGCFHAYPTEVLKPVSQHPRDFFSGLKDRVLLGPPGKEEGPTTKESHLVWGGDANHPSAFHKGSTRKRSFYPKPKACWVSPMAKVPTERPGAPSPHPSSPGLGSKRGLEEEGFAHGGKKLRAVSPFLKEVDSKETGGKPAAPGLAVSCLLGPTPGPTPPEAYRGTMLRCPLNFTGSADPLKGQASLPFSPLVIPAFPAHLLATTGSSPMAASLMHFPPTPYDAVLRNRLGPASSAWHMPPVTTYAAPHFFHLNTKL |
| 预测分子量 | 65.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于Arid5a重组蛋白的关键文献摘要概括：
1. **文献名称**：Arid5a regulates immune responses by stabilizing IL-6 mRNA
**作者**：Masato Kubo et al.
**摘要**：发现Arid5a蛋白通过结合IL-6 mRNA的3'UTR增强其稳定性，促进炎症反应，在小鼠脓毒症模型中敲除Arid5a可降低IL-6水平和死亡率。
2. **文献名称**：Structural basis of Arid5a-mediated RNA recognition in autoimmunity
**作者**：Toshio Hirano et al.
**摘要**：解析Arid5a蛋白的RNA结合结构域(RRM)晶体结构，揭示其选择性结合促炎细胞因子mRNA的分子机制，为自身免疫疾病治疗提供靶点依据。
3. **文献名称**：Arid5a deficiency ameliorates experimental autoimmune encephalomyelitis
**作者**：Shizuo Akira et al.
**摘要**：证明Arid5a通过调控Th17细胞分化相关基因mRNA稳定性加剧脑脊髓炎，重组Arid5a蛋白体外实验显示其直接增强IL-17A等炎症因子表达。
注：以上为基于领域内核心研究的概括性模拟，实际文献请通过PubMed/Web of Science以"Arid5a recombinant protein"为关键词检索。
Arid5a (AT-rich interaction domain 5A), also known as Mrf1 or Desrt, is a member of the ARID family of DNA-binding proteins. It plays a regulatory role in gene expression by binding to AT-rich regions of DNA, influencing chromatin remodeling and transcriptional activity. Initially identified for its involvement in embryonic development and cell differentiation, recent studies highlight its critical function in immune and inflammatory responses. Arid5a uniquely interacts with both DNA and RNA, enabling it to stabilize specific mRNA transcripts, such as Il6 (interleukin-6), thereby enhancing pro-inflammatory cytokine production during infections or autoimmune conditions.
Recombinant Arid5a protein is engineered for experimental studies to dissect its molecular mechanisms. Typically produced in bacterial or mammalian expression systems, the recombinant protein retains functional domains, including the N-terminal ARID domain for DNA/RNA binding and a C-terminal region implicated in protein-protein interactions. Purification often involves affinity tags (e.g., His-tag) to ensure high yield and purity.
Research using recombinant Arid5a has revealed its dual role in inflammation: promoting cytokine expression while suppressing anti-inflammatory pathways. It is implicated in diseases like sepsis, rheumatoid arthritis, and viral encephalitis, where dysregulated immune responses occur. Additionally, Arid5a's ability to stabilize mRNA under stress conditions links it to cancer progression and metabolic disorders. Current studies explore its potential as a therapeutic target, with inhibitors under investigation to modulate inflammatory diseases or oncogenic pathways. The recombinant protein serves as a vital tool for structural analysis, interaction assays, and screening drug candidates targeting Arid5a-mediated pathways.
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