| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DPYSL2 |
| Uniprot No | Q16555 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-572aa |
| 氨基酸序列 | MSYQGKKNIPRITSDRLLIKGGKIVNDDQSFYADIYMEDGLIKQIGENLIVPGGVKTIEAHSRMVIPGGIDVHTRFQMPDQGMTSADDFFQGTKAALAGGTTMIIDHVVPEPGTSLLAAFDQWREWADSKSCCDYSLHVDISEWHKGIQEEMEALVKDHGVNSFLVYMAFKDRFQLTDCQIYEVLSVIRDIGAIAQVHAENGDIIAEEQQRILDLGITGPEGHVLSRPEEVEAEAVNRAITIANQTNCPLYITKVMSKSSAEVIAQARKKGTVVYGEPITASLGTDGSHYWSKNWAKAAAFVTSPPLSPDPTTPDFLNSLLSCGDLQVTGSAHCTFNTAQKAVGKDNFTLIPEGTNGTEERMSVIWDKAVVTGKMDENQFVAVTSTNAAKVFNLYPRKGRIAVGSDADLVIWDPDSVKTISAKTHNSSLEYNIFEGMECRGSPLVVISQGKIVLEDGTLHVTEGSGRYIPRKPFPDFVYKRIKARSRLAELRGVPRGLYDGPVCEVSVTPKTVTPASSAKTSPAKQQAPPVRNLHQSGFSLSGAQIDDNIPRRTTQRIVAPPGGRANITSLG |
| 预测分子量 | 64.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DPYSL2重组蛋白的3篇模拟参考文献及其摘要概括：
1. **标题**：Recombinant DPYSL2 regulates axonal growth via microtubule dynamics in neuronal cells
**作者**：T. Yamashita et al.
**摘要**：该研究通过在大肠杆菌中表达并纯化重组DPYSL2蛋白，证实其通过调节微管稳定性促进神经元轴突生长，揭示了其在神经发育中的关键作用。
2. **标题**：Crystal structure of human DPYSL2 reveals a phosphorylation-dependent functional switch
**作者**：K. R. Ariga et al.
**摘要**：本文解析了DPYSL2重组蛋白的晶体结构，发现其功能受磷酸化调控，磷酸化修饰可改变蛋白构象并影响与Semaphorin信号通路相关蛋白的相互作用。
3. **标题**：DPYSL2 as a biomarker in Alzheimer's disease: Recombinant protein-based autoantibody detection
**作者**：M. L. Campos et al.
**摘要**：研究利用重组DPYSL2蛋白检测阿尔茨海默病患者血清中的自身抗体，表明其可能作为疾病早期诊断的潜在生物标志物。
注：以上文献为示例性内容，实际研究中请通过学术数据库(如PubMed、Google Scholar)检索真实文献。
**Background of DPYSL2 Recombinant Protein**
DPYSL2 (Dihydropyrimidinase-like 2), also known as CRMP2 (Collapsin Response Mediator Protein 2), is a cytosolic phosphoprotein encoded by the *DPYSL2* gene. It belongs to the CRMP family, which plays critical roles in neuronal development, including axon guidance, dendritic patterning, and neuronal migration. DPYSL2 is highly expressed in the nervous system and interacts with microtubules, cytoskeletal components, and signaling molecules to regulate neuronal polarity and growth cone dynamics.
Structurally, DPYSL2 contains an N-terminal helical domain and a C-terminal hydrolase-like domain, though it lacks enzymatic activity. Its function is modulated by post-translational modifications, particularly phosphorylation, which influences its interactions with tubulin and other partners. Dysregulation of DPYSL2 has been implicated in neurodevelopmental disorders (e.g., schizophrenia, Alzheimer’s disease), cancer progression, and neuropathic pain, highlighting its biomedical relevance.
Recombinant DPYSL2 protein is produced in vitro using expression systems like *E. coli* or mammalian cell lines, enabling studies on its molecular mechanisms and therapeutic potential. It is purified via affinity tags (e.g., His-tag) and validated for functional assays, such as microtubule binding or neurite outgrowth experiments. Researchers utilize DPYSL2 recombinant protein to investigate its role in Semaphorin/Wnt signaling pathways, screen drug candidates targeting CRMP2-associated pathologies, or develop diagnostic biomarkers. Its application spans neuroscience, oncology, and drug discovery, underscoring its versatility as a tool for both basic and translational research.
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