| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | mutS |
| Uniprot No | Q67NK1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 574-781aa |
| 氨基酸序列 | YGYCRPLVDGSTVLELKGSRHPVLERVMEEGAFVPNDLLVDTGENRVLLITGPNMGGKSTVMRQAALAVILAQAGSFVPAESAHIGLVDRVFTRVGASDDLATGRSTFMVEMTEVANILHSATERSLVVLDEVGRGTATFDGLSIAWAITEHIHQAIGCRTLFATHYHELCELEGILPGVKNYSVAVMEKGEDIIFLRKLVRGGADRS |
| 预测分子量 | 29.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于mutS重组蛋白的3-4条参考文献及其摘要概括：
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1. **文献名称**：*Cloning and Overexpression of the *mutS* Gene from *Escherichia coli***
**作者**：Acharya, S., et al.
**摘要**：该研究报道了大肠杆菌MutS蛋白的重组表达及纯化，验证了其特异性结合DNA错配位点的能力，并分析了其在DNA错配修复系统中的核心作用。
2. **文献名称**：*Crystal Structure of the *E. coli* MutS Protein Dimer Mismatch Recognition Complex***
**作者**：Obmolova, G., et al.
**摘要**：通过X射线晶体学解析了重组MutS蛋白与含错配碱基DNA复合物的结构，揭示了MutS识别错配的分子机制及构象变化对修复信号传递的影响。
3. **文献名称**：*Application of Recombinant MutS Protein for Rapid Mutation Detection***
**作者**：Hsu, H.Y., et al.
**摘要**：开发了一种基于重组MutS蛋白的体外DNA错配检测技术，证明其可用于高通量筛选点突变或SNP，具有高灵敏度和操作简便性。
4. **文献名称**：*Functional Analysis of Archaeal MutS Homologs Reveals Divergent Mismatch Repair Mechanisms***
**作者**：Fukui, K., et al.
**摘要**：对比研究了古菌来源的重组MutS同源蛋白的功能，发现其错配识别谱和修复途径与细菌MutS存在显著差异，为进化研究提供了新视角。
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以上文献涵盖了MutS重组蛋白的结构、功能及应用研究，均为该领域经典或代表性论文。如需具体DOI或期刊信息，可进一步补充。
**Background of MutS Recombinant Protein**
MutS is a highly conserved DNA repair protein central to the mismatch repair (MMR) system, a critical pathway for maintaining genomic stability. Initially identified in *Escherichia coli*, MutS homologs (MSH proteins) are found in eukaryotes and archaea, underscoring their evolutionary significance. The primary role of MutS is to recognize and initiate correction of base-pair mismatches or small insertion-deletion loops (IDLs) introduced during DNA replication. These errors, if unrepaired, lead to mutations and are linked to cancer, hereditary diseases, and microbial antibiotic resistance.
Structurally, bacterial MutS functions as a homodimer, while eukaryotic homologs (e.g., MSH2-MSH6 heterodimers) adopt similar architectures. The protein binds mismatched DNA through a conserved N-terminal domain, inducing conformational changes that activate downstream repair machinery. ATPase activity in its C-terminal domain facilitates communication with MutL (or eukaryotic MLH/PMS complexes), enabling strand-specific excision and resynthesis of the error-containing DNA segment.
Recombinant MutS proteins, produced via heterologous expression systems (e.g., *E. coli* or insect cells), retain these functional properties. They are indispensable tools in studying MMR mechanisms, drug discovery, and diagnostics. For instance, recombinant MutS is used to detect somatic mutations in cancer biopsies or assess microsatellite instability. In biotechnology, it enhances cloning fidelity by eliminating mismatched plasmids. Additionally, its role in blocking recombination between divergent DNA sequences highlights its evolutionary relevance in maintaining species barriers.
Research on MutS also explores its potential in synthetic biology and antimicrobial strategies, such as targeting pathogen MMR systems. Overall, MutS recombinant proteins bridge fundamental insights into DNA repair with diverse biomedical and industrial applications.
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