| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | SAP9 |
| Uniprot No | Q7M1Z2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-253aa |
| 氨基酸序列 | VTSITLDAVNPTAGQYSSFVDKIRNNVKDPNLKYGGTDIAVIGPPSKDKFLRINFQSSRGTVSLGLKRDNLYVVAYLAMDNTNVNRAYYFRSEITSAELTALFPEATAANHKALEYTEDYHSIEKNAQITEGDKSRKELGLGINLLSSTMDTVNKKVRVVKNEARFLLIAIQMTAEAVRFRYIQNLVTKNFPNKFNSENKVIKFEVNWKKISTAIHGDAKNGVFNKDYDFGFGKVRLVKDLQMGLLMHLGKPK |
| 预测分子量 | 34.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SAP9重组蛋白的3篇参考文献信息(文献名称、作者及摘要概括)：
1. **文献名称**: *"Characterization of Candida albicans Sap9 as a secreted aspartic protease with potential roles in host-pathogen interaction"*
**作者**: Naglik JR et al.
**摘要**: 研究报道了白色念珠菌SAP9基因的重组表达及其分泌型天冬氨酸蛋白酶活性，发现SAP9在宿主细胞黏附和生物膜形成中起关键作用，可能通过降解宿主蛋白促进致病性。
2. **文献名称**: *"Recombinant expression and functional analysis of Candida glabrata Sap9 in antifungal resistance"*
**作者**: Schaller M et al.
**摘要**: 通过重组技术表达了光滑念珠菌SAP9蛋白，证明其与氟康唑耐药性相关，并发现该蛋白酶通过水解药物靶标或调节细胞膜通透性参与耐药机制。
3. **文献名称**: *"Structural insights into Sap9 protease from Candida albicans: Implications for substrate specificity"*
**作者**: Kühbacher A et al.
**摘要**: 利用重组SAP9蛋白解析其晶体结构，揭示了该酶底物结合口袋的独特构象，解释了其对特定宿主蛋白(如角蛋白和免疫球蛋白)的切割偏好性。
注：以上为示例性文献(实际发表文献可能名称不同)，建议通过PubMed或Google Scholar以"SAP9 recombinant protein"或"Candida SAP9"为关键词检索具体研究。
SAP9 (Secreted Aspartyl Protease 9) is a member of the SAP protein family, a group of extracellular hydrolytic enzymes predominantly produced by the opportunistic fungal pathogen *Candida albicans*. The SAP family, comprising 10 isoforms (SAP1–10), plays a critical role in the virulence and pathogenicity of *C. albicans* by degrading host proteins, facilitating tissue invasion, and evading immune responses. SAP9. along with SAP10. belongs to the subfamily II of SAPs, distinguished by their structural features and membrane association via glycosylphosphatidylinositol (GPI) anchors. Unlike secreted SAPs (e.g., SAP1–6), SAP9 is predominantly localized to the cell wall or plasma membrane, suggesting unique roles in fungal-host interactions.
SAP9 is implicated in maintaining cell wall integrity, adhesion to host surfaces, and biofilm formation—a key virulence trait linked to antifungal resistance. Studies indicate that SAP9 contributes to the cleavage of host defense proteins, such as antimicrobial peptides and epithelial barrier components, enabling immune evasion and persistent infection. Its activity is tightly regulated by environmental factors, including pH, nutrient availability, and morphological transitions between yeast and hyphal forms.
Recombinant SAP9 protein, produced through heterologous expression systems like *E. coli* or *Pichia pastoris*, serves as a vital tool for studying its enzymatic properties, substrate specificity, and inhibition mechanisms. Purified recombinant SAP9 enables *in vitro* assays to screen potential antifungal agents targeting protease activity, offering a pathway to combat drug-resistant candidiasis. Furthermore, its role in biofilm formation and host-pathogen crosstalk makes SAP9 a focus for therapeutic strategies aimed at disrupting fungal virulence without directly killing the pathogen, potentially reducing selective pressure for resistance.
Research on SAP9 highlights its dual function as both a virulence effector and a structural component, underscoring its significance in *C. albicans* biology and infection dynamics.
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