| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SERPINA3-2 |
| Uniprot No | A2I7M9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-411aa |
| 氨基酸序列 | LPENVVVKDQHRRVDGHTLASSNTDFAFSLYKQLALKNPNKNVILSPLSVSIALAFLSLGARGSTLTEILEGLKFNLTEIQEKEIHHSFQHLLQALNQPSNQLQLSVGNAMFVQEELKLLDKFIEDAQVLYSSEAFPTNFRDSEAARSLINDYVKNKTQGKIEELFKYLSPRTELVLVNYIYFKAQWKTPFDPKHTEQAEFHVSDNKTVEVPMMTLDLETPYFRDEELGCTLVELTYTSNDSALFILPDEGKMRDLEAKLTPETLTRWRNSLQPRRIHELYLPKFSIKSNYELNDILSQLGIRKIFANADLSGITGTADLVVSQVVHGAALDVDEEGTEGVAATGIGIERTFLRIIVRVNRPFLIAVVLKDTQSIIFLGKVTNPSEA |
| 预测分子量 | 45.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SERPINA3-2重组蛋白的3篇代表性文献的简要信息(注：文献为模拟示例，实际需查阅数据库确认)：
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1. **文献名称**：Recombinant SERPINA3-2 Protein Attenuates Neuroinflammation in Alzheimer's Disease Models
**作者**：Tampi, R.R., et al. (2021)
**摘要**：研究成功在大肠杆菌中表达并纯化SERPINA3-2重组蛋白，发现其能抑制β-淀粉样蛋白诱导的小胶质细胞炎症反应，提示其在阿尔茨海默病中的潜在治疗作用。
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2. **文献名称**：Expression and Functional Characterization of Human SERPINA3-2 in Hepatocellular Carcinoma
**作者**：Zhang, Y., et al. (2019)
**摘要**：通过哺乳动物细胞系统重组表达SERPINA3-2蛋白，证实其通过调节蛋白酶活性抑制肝癌细胞侵袭转移，为肝癌靶向治疗提供新思路。
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3. **文献名称**：SERPINA3-2 as a Novel Acute-phase Protein: Insights from Recombinant Protein-based Studies
**作者**：Law, R.H., et al. (2020)
**摘要**：利用重组SERPINA3-2蛋白分析其在炎症急性期反应中的调控机制，发现其血清水平与CRP显著相关，可能作为炎症性疾病的新型生物标志物。
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(注：以上文献信息为基于领域知识的模拟生成，实际文献需通过PubMed、Web of Science等平台检索。)
SERPINA3-2. also known as alpha-1-antichymotrypsin (AACT), is a member of the SERPIN (serine protease inhibitor) superfamily. This glycoprotein is encoded by the SERPINA3 gene located on human chromosome 14q32.13. Naturally produced in the liver, it circulates in blood plasma and functions as an acute-phase reactant, with levels increasing during inflammation. Its primary role involves inhibiting serine proteases like cathepsin G and mast cell chymase, modulating proteolytic activity in immune responses and tissue homeostasis.
Recombinant SERPINA3-2 protein is engineered through molecular cloning techniques, typically expressed in mammalian or bacterial systems to ensure proper folding and post-translational modifications. The protein's structure features a conserved SERPIN domain with reactive center loops critical for protease interaction. Researchers utilize the recombinant form to study its biological functions beyond innate immunity, including emerging roles in cancer progression, neurodegenerative diseases (e.g., Alzheimer's disease where it colocalizes with amyloid plaques), and chronic inflammatory conditions.
Recent studies highlight its dual nature – while exhibiting anti-protease activity, SERPINA3-2 may acquire gain-of-function properties in pathological microenvironments. Pharmaceutical interest focuses on its therapeutic potential for regulating excessive protease activity or developing biomarkers for disease monitoring. However, challenges remain in understanding its context-dependent functions and optimizing recombinant production for clinical applications. Current research continues to explore its complex interplay in cellular processes and disease mechanisms.
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