| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | pntA |
| Uniprot No | P07001 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-510aa |
| 氨基酸序列 | MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKAGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTEEKCTCSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKN |
| 预测分子量 | 54,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于pntA重组蛋白的模拟参考文献示例，涵盖不同研究方向：
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1. **文献名称**：*"Cloning and Heterologous Expression of the pntA Gene in Escherichia coli for NADPH Regeneration"*
**作者**：Smith, J. et al.
**摘要**：本研究报道了通过PCR扩增大肠杆菌pntA基因，构建重组质粒并在宿主中高效表达。纯化的重组蛋白显示转氢酶活性，证实其在NADPH再生系统中的潜在应用。
2. **文献名称**：*"Structural and Functional Characterization of Recombinant pntA-Encoded Transhydrogenase"*
**作者**：Zhang, L. & Kumar, R.
**摘要**：通过X射线晶体学解析pntA重组蛋白的三维结构，揭示其底物结合域和催化机制。酶活实验表明该蛋白在pH 7.5时活性最高，为代谢工程改造提供依据。
3. **文献名称**：*"Enhancing Metabolic Flux in Saccharomyces cerevisiae by pntA Recombinant Protein Expression"*
**作者**：Chen, H. et al.
**摘要**：在酿酒酵母中异源表达pntA重组蛋白，显著提升NADPH水平，促进脂肪酸合成。研究证明该策略可用于优化微生物细胞工厂的产物合成效率。
4. **文献名称**：*"Kinetic Analysis of Recombinant pntA Transhydrogenase and Its Role in Oxidative Stress Response"*
**作者**：Martinez, D. et al.
**摘要**：系统测定pntA重组蛋白的酶动力学参数(Km/Vmax)，并发现其表达上调可增强大肠杆菌对氧化应激的抗性，提示其在细胞代谢平衡中的关键作用。
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注：以上文献为模拟示例，实际引用需通过学术数据库检索真实研究。
**Background of pntA Recombinant Protein**
The *pntA* gene encodes the E1 component (pyruvate dehydrogenase) of the pyruvate dehydrogenase complex (PDHC), a critical enzyme system in cellular metabolism. PDHC catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA, linking glycolysis to the tricarboxylic acid (TCA) cycle. This reaction is essential for aerobic energy production and biosynthetic pathways. In bacteria like *Escherichia coli*, *pntA* is part of the *pdh* operon, which also includes *aceE* and *aceF*, encoding the E1. E2. and E3 subunits, respectively. Dysregulation of PDHC activity is associated with metabolic disorders in humans and impaired bacterial growth under certain conditions.
Recombinant pntA protein is produced via heterologous expression systems (e.g., *E. coli* or yeast) to study its structure, enzymatic activity, and regulatory mechanisms. Researchers often clone the *pntA* gene into expression vectors under inducible promoters, enabling high-yield production of the purified protein. This recombinant approach allows for detailed biochemical analyses, including substrate specificity, cofactor interactions (e.g., thiamine pyrophosphate dependency), and inhibition studies.
Applications of pntA recombinant protein span metabolic engineering, drug discovery, and enzyme replacement therapies. It serves as a tool to investigate microbial metabolism, optimize biofuel production pathways, or screen for antimicrobial agents targeting bacterial PDHC. Additionally, its study contributes to understanding analogous human enzymes, aiding research on diseases like pyruvate dehydrogenase deficiency. The recombinant protein’s scalability and purity make it valuable for both academic and industrial research.
In summary, pntA recombinant protein is a key reagent for exploring central carbon metabolism, microbial physiology, and therapeutic interventions, bridging fundamental science with biotechnological applications.
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