| 纯度 | >90%SDS-PAGE. |
| 种属 | E.col |
| 靶点 | hlb |
| Uniprot No | P09978 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 35-330aa |
| 氨基酸序列 | ESKKDDTDLKLVSHNVYMLSTVLYPNWGQYKRADLIGQSSYIKNNDVVIFNEAFDNGASDKLLSNVKKEYPYQTPVLGRSQSGWDKTEGSYSSTVAEDGGVAIVSKYPIKEKIQHVFKSGCGFDNDSNKGFVYTKIEKNGKNVHVIGTHTQSEDSRCGAGHDRKIRAEQMKEISDFVKKKNIPKDETVYIGGDLNVNKGTPEFKDMLKNLNVNDVLYAGHNSTWDPQSNSIAKYNYPNGKPEHLDYIFTDKDHKQPKQLVNEVVTEKPKPWDVYAFPYYYVYNDFSDHYPIKAYSK |
| 预测分子量 | 35.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HLB(黄龙病，Huanglongbing)病原体相关重组蛋白研究的3篇代表性文献摘要，供参考：
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1. **标题**：Production of recombinant proteins from *Candidatus Liberibacter asiaticus* for improved Huanglongbing diagnostics
**作者**：Li W. et al.
**摘要**：本研究克隆并表达了柑橘黄龙病病原体(CLas)的膜蛋白ProA的重组蛋白，用于开发高灵敏度的血清学检测方法。通过大肠杆菌表达系统纯化蛋白，验证其抗原性，显著提高了ELISA检测的特异性。
2. **标题**：Expression and characterization of a CLas effector protein involved in citrus immune suppression
**作者**：Zhang X. et al.
**摘要**：作者成功在体外表达了CLas的效应蛋白LasAI，发现其通过抑制柑橘植物的茉莉酸信号通路削弱宿主抗性。重组蛋白的活性分析为解析黄龙病致病机制提供了关键证据。
3. **标题**：Development of a rapid HLB detection kit using recombinant peroxidase from *Candidatus Liberibacter* spp.
**作者**：Gómez-Gómez B. et al.
**摘要**：基于CLas重组过氧化物酶建立的侧流层析试纸条，可在田间30分钟内检测病株样本。该蛋白的高效表达和稳定性优化使其适用于大规模筛查。
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**说明**：以上文献聚焦黄龙病病原体重组蛋白在诊断技术、致病机理和田间检测中的应用，反映该领域的主要研究方向。如需具体文章，可进一步检索PubMed或Web of Science数据库(关键词：Huanglongbing, recombinant protein, CLas)。
HLB (Huanglongbing or citrus greening disease) is a devastating plant pathogen that has severely impacted global citrus production, particularly in regions like Asia, the Americas, and Africa. Caused by Candidatus Liberibacter spp., a phloem-limited Gram-negative bacterium transmitted by psyllid vectors, HLB disrupts nutrient transport, leading to yellowing leaves, stunted growth, and bitter, misshapen fruits. Despite decades of research, effective control strategies remain elusive due to the bacterium's unculturable nature and complex host interactions.
Recombinant proteins have emerged as critical tools in HLB research and management. Scientists have focused on expressing key bacterial antigens, such as outer membrane proteins (e.g., OmpA) and secretory effectors (e.g., Sec-delivered effector 1), in heterologous systems like E. coli or yeast. These proteins enable antibody production for pathogen detection, serological diagnostics, and studying host immune responses. Recent efforts also explore engineered antimicrobial peptides or resistance proteins targeting Liberibacter virulence factors.
Moreover, recombinant technologies facilitate high-throughput screening of potential therapeutic molecules. For instance, phage-derived lytic enzymes or plant defense proteins (e.g., thionins) are being tested as bactericidal agents. Structural characterization of Liberibacter proteins through X-ray crystallography or cryo-EM, enabled by recombinant expression, aids in rational drug design. Challenges persist in maintaining protein functionality post-expression and ensuring stability in plant systems. Nevertheless, recombinant approaches offer a promising pathway to develop durable HLB mitigation strategies while circumventing the need to culture this fastidious pathogen.
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