| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | NDK1 |
| Uniprot No | P39207 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-149aa |
| 氨基酸序列 | MEQTFIMIKPDGVQRGLIGEVICRFEKKGFTLKGLKLISVERSFAEKHYEDLSSKSFFSGLVDYIVSGPVVAMIWEGKNVVLTGRKIIGATNPAASEPGTIRGDFAIDIGRNVIHGSDSVESARKEIALWFPDGPVNWQSSVHPWVYET |
| 预测分子量 | 20.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NDK1重组蛋白的示例参考文献(内容为示例，实际文献需根据具体研究检索确认)：
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1. **《Expression and Purification of Recombinant NDK1 in Escherichia coli》**
*Zhang et al., 2015*
摘要：研究通过大肠杆菌系统高效表达并纯化NDK1重组蛋白，优化了诱导条件与纯化步骤(如His标签亲和层析)，并验证其具有核苷二磷酸激酶活性，为后续功能研究奠定基础。
2. **《Structural Insights into NDK1 by X-ray Crystallography》**
*Li et al., 2018*
摘要：解析了NDK1重组蛋白的晶体结构(分辨率2.1 Å)，揭示了其六聚体构象及活性位点关键氨基酸残基，为阐明其催化机制提供结构生物学依据。
3. **《NDK1 Promotes Tumor Cell Migration via Regulation of F-actin Dynamics》**
*Wang et al., 2020*
摘要：发现重组NDK1通过调控细胞骨架蛋白相互作用促进肿瘤细胞迁移，体外实验表明其通过磷酸化GDP为GTP影响Rho家族蛋白活性，进而驱动侵袭转移。
4. **《Functional Characterization of NDK1 in Arabidopsis Stress Response》**
*Chen et al., 2017*
摘要：利用重组NDK1蛋白分析其在拟南芥中的功能，证实其通过调节细胞能量代谢参与干旱胁迫响应，过表达植株表现出更强的抗逆性。
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提示：实际文献需通过PubMed、Google Scholar等平台以“NDK1 recombinant protein”“Nucleoside diphosphate kinase 1”等关键词检索，并筛选目标研究方向(如表达、结构、疾病或植物学应用)。
**Background of NDK1 Recombinant Protein**
Nucleoside diphosphate kinase 1 (NDK1), also known as NME1 or NM23-H1. is a conserved enzyme encoded by the *NME1* gene. It belongs to the nucleoside diphosphate kinase (NDK) family, which catalyzes the transfer of phosphate groups between nucleoside triphosphates (NTPs) and diphosphates (NDPs), maintaining nucleotide balance critical for DNA/RNA synthesis, energy metabolism, and cellular signaling. NDK1 is particularly notable for its multifunctional roles beyond its phosphotransferase activity, including involvement in cell proliferation, differentiation, metastasis suppression, and apoptosis regulation.
Recombinant NDK1 protein is engineered using genetic cloning techniques, where the *NME1* gene is expressed in heterologous systems like *E. coli* or mammalian cell lines (e.g., HEK293). This allows large-scale production of purified, bioactive NDK1 for research and therapeutic applications. The recombinant form often includes tags (e.g., His-tag) for simplified purification and detection.
Studies on NDK1 have highlighted its dual role in cancer biology—acting as a metastasis suppressor in certain contexts while promoting tumor progression in others. Its interaction with proteins like GTPases and histones underscores its regulatory influence on cytoskeletal dynamics, gene expression, and DNA repair. Additionally, NDK1 is implicated in microbial infections, as pathogenic bacteria secrete homologs to manipulate host cell processes.
Research applications of recombinant NDK1 include enzymatic assays, structural studies, drug discovery (e.g., targeting nucleotide metabolism in cancer), and exploring its metastasis-suppressive mechanisms. Its therapeutic potential is being investigated in conditions like metastatic cancers and neurodegenerative diseases. However, context-dependent functions and post-translational modifications necessitate careful characterization of recombinant NDK1 to ensure biological relevance in experimental models.
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