| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | DOG1 |
| Uniprot No | P38774 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-246aa |
| 氨基酸序列 | MAEFSADLCLFDLDGTIVSTTVAAEKAWTKLCYEYGVDPSELFKHSHGARTQEVLRRFFPKLDDTDNKGVLALEKDIAHSYLDTVSLIPGAENLLLSLDVDTETQKKLPERKWAIVTSGSPYLAFSWFETILKNVGKPKVFITGFDVKNGKPDPEGYSRARDLLRQDLQLTGKQDLKYVVFEDAPVGIKAGKAMGAITVGITSSYDKSVLFDAGADYVVCDLTQVSVVKNNENGIVIQVNNPLTRA |
| 预测分子量 | 29.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DOG1重组蛋白的3篇代表性文献示例(内容为模拟概括，建议通过学术数据库查询具体文献)：
1. **文献名称**：*Expression and Purification of Recombinant DOG1 Protein for Antibody Production*
**作者**：Smith A, et al.
**摘要**：研究通过大肠杆菌系统表达重组DOG1蛋白，优化纯化流程并制备多克隆抗体，验证其在胃肠道间质瘤(GIST)免疫组化诊断中的应用效果。
2. **文献名称**：*Functional Characterization of DOG1/ANO1 Chloride Channel in Cancer Cells*
**作者**：Lee JH, et al.
**摘要**：利用哺乳动物细胞系表达重组DOG1蛋白，证实其作为钙激活氯离子通道的功能，并探讨其在肿瘤细胞迁移和增殖中的调控机制。
3. **文献名称**：*DOG1 Recombinant Protein as a Biomarker in Gastrointestinal Stromal Tumors*
**作者**：Garcia-Rico E, et al.
**摘要**：通过真核表达系统获得高纯度DOG1重组蛋白，分析其与临床GIST样本的结合特异性，提出其在分子分型和靶向治疗中的潜在价值。
**提示**：实际文献需通过PubMed、Web of Science等平台检索关键词“DOG1 recombinant protein”“ANO1 expression”获取，部分研究可能涉及基因克隆、结构解析或诊断试剂开发。
**Background of DOG1 Recombinant Protein**
DOG1 (Discovered On Gastrointestinal stromal tumor 1), also known as ANO1 or TMEM16A, is a calcium-activated chloride channel protein encoded by the *ANO1* gene. It belongs to the anoctamin family (TMEM16) and plays a critical role in regulating ion transport, cell volume, and epithelial secretion. DOG1 was initially identified as a biomarker for gastrointestinal stromal tumors (GISTs), where its expression is strongly associated with KIT mutations, aiding in diagnosis and differential classification of these tumors.
Structurally, DOG1 contains eight transmembrane domains and requires calcium binding for channel activation. Its function extends beyond tumors, contributing to physiological processes such as smooth muscle contraction, neuronal excitability, and salivary/pancreatic fluid secretion. Dysregulation of DOG1 has been implicated in pathologies, including cystic fibrosis, hypertension, and cancers (e.g., head-neck squamous cell carcinoma, breast cancer).
Recombinant DOG1 protein is produced using heterologous expression systems (e.g., mammalian cells, bacteria) to study its biophysical properties, ion channel activity, and interactions with pharmacological modulators. Purified recombinant DOG1 enables *in vitro* assays for drug discovery, particularly for targeting chloride channel-related disorders. It also serves as an antigen for antibody development, enhancing diagnostic accuracy in histopathology.
Research using recombinant DOG1 has advanced understanding of its role in tumorigenesis and cellular signaling, offering potential therapeutic avenues. However, challenges remain in elucidating its precise regulatory mechanisms and tissue-specific functions. Overall, DOG1 recombinant protein remains a vital tool for both basic research and clinical applications in oncology and channelopathy studies.
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