| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KAE1 |
| Uniprot No | Q9NPF4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-335aa |
| 氨基酸序列 | MPAVLGFEGSANKIGVGVVRDGKVLANPRRTYVTPPGTGFLPGDTARHHRAVILDLLQEALTESGLTSQDIDCIAYTKGPGMGAPLVSVAVVARTVAQLWNKPLVGVNHCIGHIEMGRLITGATSPTVLYVSGGNTQVIAYSEHRYRIFGETIDIAVGNCLDRFARVLKISNDPSPGYNIEQMAKRGKKLVELPYTVKGMDVSFSGILSFIEDVAHRMLATGECTPEDLCFSLQETVFAMLVEITERAMAHCGSQEALIVGGVGCNVRLQEMMATMCQERGARLFATDERFCIDNGAMIAQAGWEMFRAGHRTPLSDSGVTQRYRTDEVEVTWRD |
| 预测分子量 | 63.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KAE1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*Structure and Function of the Kae1 Protein in the KEOPS Complex*
**作者**：Daugeron, M. C., et al.
**摘要**：该研究解析了Kae1作为KEOPS复合体核心组分的结构与功能，发现其通过重组蛋白技术在大肠杆菌中表达并纯化，揭示了Kae1在tRNA的t⁶A修饰中的关键催化作用，并阐明其与复合体其他成员的相互作用机制。
2. **文献名称**：*Biochemical Characterization of Recombinant Kae1 from Archaea*
**作者**：El Yacoubi, B., et al.
**摘要**：本文报道了古菌来源的Kae1重组蛋白的表达与纯化，通过X射线晶体学解析其三维结构，并验证其ATP酶活性及在tRNA修饰中的底物结合特性，为理解Kae1的进化保守性提供依据。
3. **文献名称**：*Functional Complementation of Bacterial Kae1 Reveals Its Essential Role in Cell Viability*
**作者**：Srinivasan, M., et al.
**摘要**：研究利用重组Kae1蛋白进行功能回补实验，证明其在细菌生长中的必需性，并通过体外酶活实验表明Kae1的缺失导致t⁶A修饰缺陷，从而影响翻译保真性和细胞存活。
(注：以上文献信息为示例性质，实际引用时需核实具体文献内容及作者信息。)
KAE1 (Kinase-Associated Endopeptidase 1) is a conserved protein involved in critical cellular processes across archaea, bacteria, and eukaryotes. It is a core component of the KEOPS (Kinase, Endopeptidase, and Other Proteins of Small size) complex, which plays an essential role in the biosynthesis of the tRNA modification N6-threonylcarbamoyladenosine (t⁶A). This modification ensures translational fidelity by stabilizing codon-anticodon interactions during protein synthesis. Structural and functional studies reveal that KAE1 exhibits both endopeptidase and ATPase activities, though its precise catalytic mechanisms remain under investigation.
In humans, KAE1 (also known as OSGEP) is implicated in DNA repair, telomere maintenance, and mitotic progression. Dysregulation of KAE1 or the KEOPS complex is linked to genetic disorders such as Galloway-Mowat syndrome, a severe neurodevelopmental condition, and has been associated with cancer progression due to its role in genome stability. Recombinant KAE1 protein is typically produced using heterologous expression systems (e.g., *E. coli* or insect cells*) to enable biochemical characterization, structural analysis (e.g., X-ray crystallography), and inhibitor screening. Its study provides insights into conserved molecular pathways and potential therapeutic targets for diseases linked to tRNA modification defects or genomic instability. Research on KAE1 also intersects with antimicrobial drug development, as bacterial-specific features of the KEOPS complex may serve as targets for novel antibiotics.
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