| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | yscF |
| Uniprot No | Q01247 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-87aa |
| 氨基酸序列 | MSNFSGFTKGNDIADLDAVAQTLKKPADDANKAVNDSIAALKDTPDNPALLADLQHSINKWSVIYNISSTIVRSMKDLMQGILQKFP |
| 预测分子量 | 9,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于YscF重组蛋白的3篇参考文献示例：
1. **标题**: "Recombinant YscF Protein of Yersinia pestis: Purification and Crystallization"
**作者**: Smith A, et al.
**摘要**: 研究成功在大肠杆菌中重组表达并纯化YscF蛋白，利用X射线衍射分析其结构，揭示了其作为T3SS针状复合物的组装机制。
2. **标题**: "Immunogenicity of YscF in a Murine Model of Yersinia Infection"
**作者**: Chen L, et al.
**摘要**: 评估重组YscF作为疫苗候选的潜力，实验显示其能诱导特异性抗体反应，显著提高小鼠对致死剂量耶尔森菌感染的存活率。
3. **标题**: "Functional Characterization of YscF in Type III Secretion System Assembly"
**作者**: Tanaka K, et al.
**摘要**: 通过基因敲除和互补实验，证实YscF是T3SS针状结构形成的必需蛋白，重组YscF可恢复突变菌株的分泌功能及细胞毒性。
(注：以上文献为示例性质，实际引用请根据具体研究检索权威数据库。)
YscF is a key structural protein associated with the type III secretion system (T3SS) in pathogenic *Yersinia* species, including *Yersinia pestis* (the causative agent of plague) and *Yersinia pseudotuberculosis*. The T3SS is a needle-like macromolecular complex critical for bacterial pathogenesis, enabling the direct translocation of virulence effector proteins from the bacterium into host cells. YscF serves as the primary subunit of the T3SS needle, polymerizing into a hollow filament that spans the bacterial membrane and connects to the host cell during infection. This structure facilitates the delivery of cytotoxic or immunomodulatory effectors, disrupting host immune responses and promoting bacterial survival.
Recombinant YscF protein is generated through heterologous expression systems, such as *Escherichia coli*, followed by purification. Its production enables detailed structural and functional studies, including investigations into needle assembly, host-pathogen interactions, and immune evasion mechanisms. Studies have shown that YscF polymerization is tightly regulated, with chaperone proteins like YscE and YscG stabilizing monomeric YscF prior to needle assembly. Mutations or deletions in YscF often impair T3SS function, underscoring its essential role in virulence.
Beyond basic research, recombinant YscF has applications in vaccine development. As a surface-exposed component, it can elicit protective immune responses in animal models, making it a potential subunit vaccine candidate. Additionally, YscF-specific antibodies serve as diagnostic markers for *Yersinia* infections. However, challenges remain in optimizing its stability and immunogenicity for clinical use. Ongoing research focuses on resolving its atomic structure, elucidating polymerization dynamics, and engineering derivatives with enhanced therapeutic potential. Understanding YscF contributes broadly to antimicrobial strategies targeting T3SS-dependent pathogens.
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