| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SV2A |
| Uniprot No | Q7L0J3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-742aa |
| 氨基酸序列 | MEEGFRDRAAFIRGAKDIAKEVKKHAAKKVVKGLDRVQDEYSRRSYSRFEEEDDDDDFPAPSDGYYRGEGTQDEEEGGASSDATEGHDEDDEIYEGEYQGIPRAESGGKGERMADGAPLAGVRGGLSDGEGPPGGRGEAQRRKEREELAQQYEAILRECGHGRFQWTLYFVLGLALMADGVEVFVVGFVLPSAEKDMCLSDSNKGMLGLIVYLGMMVGAFLWGGLADRLGRRQCLLISLSVNSVFAFFSSFVQGYGTFLFCRLLSGVGIGGSIPIVFSYFSEFLAQEKRGEHLSWLCMFWMIGGVYAAAMAWAIIPHYGWSFQMGSAYQFHSWRVFVLVCAFPSVFAIGALTTQPESPRFFLENGKHDEAWMVLKQVHDTNMRAKGHPERVFSVTHIKTIHQEDELIEIQSDTGTWYQRWGVRALSLGGQVWGNFLSCFGPEYRRITLMMMGVWFTMSFSYYGLTVWFPDMIRHLQAVDYASRTKVFPGERVEHVTFNFTLENQIHRGGQYFNDKFIGLRLKSVSFEDSLFEECYFEDVTSSNTFFRNCTFINTVFYNTDLFEYKFVNSRLINSTFLHNKEGCPLDVTGTGEGAYMVYFVSFLGTLAVLPGNIVSALLMDKIGRLRMLAGSSVMSCVSCFFLSFGNSESAMIALLCLFGGVSIASWNALDVLTVELYPSDKRTTAFGFLNALCKLAAVLGISIFTSFVGITKAAPILFASAALALGSSLALKLPETRGQVLQ |
| 预测分子量 | 82,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SV2A重组蛋白的3篇示例参考文献，涵盖结构、功能及制备方法的研究：
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1. **文献名称**：*Structural Insights into Synaptic Vesicle Protein 2A (SV2A) Using X-ray Crystallography*
**作者**：Yao J. et al.
**摘要**：本研究通过重组表达人源SV2A蛋白的胞外结构域，利用X射线晶体学解析其三维结构，揭示了SV2A与抗癫痫药物左乙拉西坦(levetiracetam)结合的潜在位点，为靶向药物设计提供结构基础。
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2. **文献名称**：*Expression and Purification of Functional SV2A Recombinant Protein for Ligand Binding Assays*
**作者**：Dardou H. et al.
**摘要**：报道了一种在哺乳动物细胞系中高效表达并纯化SV2A重组蛋白的方法，验证其与放射性标记配体(如[³H]UCB-J)的结合活性，证明其在神经退行性疾病分子探针开发中的应用潜力。
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3. **文献名称**：*SV2A Glycosylation Modulates Synaptic Vesicle Dynamics: Insights from Recombinant Protein Mutagenesis*
**作者**：Mendoza-Torres E. et al.
**摘要**：通过构建糖基化位点突变的SV2A重组蛋白，探究糖修饰对突触囊泡运输及神经递质释放的影响，揭示了SV2A在突触可塑性中的关键作用机制。
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**备注**：以上文献为示例，实际引用时建议通过PubMed、Web of Science等数据库检索最新研究，关键词可包括“SV2A recombinant protein”、“SV2A structure”或“SV2A ligand binding”。
Synaptic vesicle glycoprotein 2A (SV2A) is a transmembrane protein predominantly localized in synaptic vesicles of neurons, playing a critical role in regulating neurotransmitter release. As a member of the SV2 family (SV2A, SV2B, SV2C), it is ubiquitously expressed in the brain and is involved in vesicle trafficking, calcium-dependent exocytosis, and maintaining synaptic function. SV2A’s structural features include 12 transmembrane domains and a glycosylated luminal domain, which may interact with key synaptic proteins like synaptotagmin. Its dysfunction has been linked to epilepsy, neurodegenerative disorders, and neuropsychiatric conditions, making it a target for both research and therapeutic development.
Recombinant SV2A proteins are engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional SV2A domains for in vitro studies. These proteins retain critical epitopes and biochemical properties, enabling investigations into SV2A’s interaction with ligands, such as the antiepileptic drug levetiracetam, which binds SV2A to modulate synaptic activity. Recombinant SV2A also facilitates structural studies (e.g., cryo-EM or X-ray crystallography) to map binding sites and understand mechanistic details at the molecular level.
In drug discovery, recombinant SV2A serves as a tool for screening small molecules or antibodies targeting SV2A-related pathways. It is also used to develop diagnostic assays, such as quantifying SV2A levels in cerebrospinal fluid as a potential biomarker for synaptic integrity in Alzheimer’s or Parkinson’s disease. Additionally, SV2A-based recombinant proteins aid in generating antibodies for immunohistochemistry or flow cytometry, advancing neurobiological research and therapeutic monitoring. Overall, SV2A recombinant proteins bridge basic neuroscience with translational applications, offering insights into synaptic biology and precision medicine strategies.
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