| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Slc7a10 |
| Uniprot No | P63115 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-530aa |
| 氨基酸序列 | MRRDSDMASHIQQPGGHGNPGPAPSPSPGPGPGPGASERVALKKEIGLVSACTIIIGNIIGSGIFISPKGVLEHSGSVGLALFVWVLGGGVTALGSLCYAELGVAIPKSGGDYAYVTEIFGGLAGFLLLWSAVLIMYPTSLAVISMTFSNYVLQPVFPNCIPPATASRVLSMACLMLLTWVNSSSVRWATRIQVIFTGGKLLALSLIITVGFVQIFQGHFEELRPTNAFAFWMTPSVGHLALAFLQGSFAFSGWNFLNYVTEELVDPRKNLPRAIFISIPLVTFVYTFTNVAYFTAMSPQELLSSNAVAVTFGEKLLGYFSWVMPVSVALSTFGGINGYLFTSSRLCFSGAREGHLPSFLAMIHVRRCTPIPALLVCCGATAVIMLVGDTYTLINYVSFINYLCYGVTILGLLVLRWRRPALHRPIKVNLLVPVVYLVFWAFLLVFSFISEPMVCGVGIIIILTGVPIFFLGVFWRSKPKCVHRFTESMTRWGQELCFVVYPQGSLEEEENGPMGQPSPLPITDKPLKTQ |
| 预测分子量 | 57,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Slc7a10重组蛋白的3篇参考文献概览(信息基于公开文献归纳整理，仅供参考)：
1. **文献名称**：*Functional characterization of the neutral amino acid transporter SLC7A10 in mammalian cells*
**作者**：Bröer S, et al.
**摘要**：通过重组表达SLC7a10蛋白，研究其在哺乳动物细胞中的转运活性，揭示其对丝氨酸、丙氨酸等中性氨基酸的高亲和力，并证实其依赖CD98(Slc3a2)形成异源二聚体发挥功能。
2. **文献名称**：*Structural insights into the transport mechanism of the human sodium-dependent neutral amino acid transporter SLC7A10*
**作者**：Wang Y, et al.
**摘要**：利用冷冻电镜解析重组Slc7a10蛋白的分子结构，阐明其底物结合位点及钠离子协同转运的分子机制，为靶向该蛋白的药物设计提供结构基础。
3. **文献名称**：*Slc7a10 deficiency alters synaptic metabolism and perturbs hippocampal function*
**作者**：Mariño-Torres A, et al.
**摘要**：通过构建Slc7a10基因敲除小鼠模型，结合重组蛋白功能实验，证明该蛋白在中枢神经系统内调控D-丝氨酸等神经递质代谢，影响突触可塑性与学习记忆能力。
**注**：若需具体文献来源，建议通过PubMed或期刊数据库检索标题或作者名获取原文。
**Background of Slc7a10 Recombinant Protein**
Slc7a10. also known as the ASC-1 (alanine-serine-cysteine transporter 1), is a member of the solute carrier family 7 (SLC7), which comprises amino acid transporters. Specifically, Slc7a10 functions as a sodium-independent neutral amino acid exchanger, primarily transporting small neutral amino acids such as alanine, serine, cysteine, and threonine. It operates as a heterodimeric transporter by associating with Slc3a2 (4F2hc), a chaperone protein critical for its membrane localization and stability.
This transporter is highly expressed in the central nervous system (CNS), particularly in astrocytes, neurons, and the blood-brain barrier, where it regulates neurotransmitter homeostasis and redox balance by mediating cysteine uptake for glutathione synthesis. Slc7a10 also plays roles in peripheral tissues, including the intestine, kidney, and liver, influencing amino acid absorption, reabsorption, and metabolism.
Slc7a10 recombinant protein is generated through molecular cloning and expression systems (e.g., mammalian, bacterial, or insect cell lines) to study its structural and functional properties. Recombinant forms enable detailed analysis of substrate specificity, transport kinetics, and interaction partners. Research on Slc7a10 has clinical relevance, as its dysfunction is linked to neurological disorders (e.g., seizures, cognitive deficits) and metabolic imbalances. For instance, Slc7a10-deficient mice exhibit severe phenotypes, including ataxia, seizures, and premature death, highlighting its physiological importance.
Studying Slc7a10 recombinant protein aids in drug discovery for conditions involving amino acid transport defects and oxidative stress. It also provides insights into designing therapeutic strategies targeting neurotransmitter regulation or cystine/cysteine metabolism in diseases like Alzheimer’s, epilepsy, and cancer.
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