| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SAG |
| Uniprot No | P10523 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-405aa |
| 氨基酸序列 | MAASGKTSKSEPNHVIFKKISRDKSVTIYLGNRDYIDHVSQVQPVDGVVLVDPDLVKGKKVYVTLTCAFRYGQEDIDVIGLTFRRDLYFSRVQVYPPVGAASTPTKLQESLLKKLGSNTYPFLLTFPDYLPCSVMLQPAPQDSGKSCGVDFEVKAFATDSTDAEEDKIPKKSSVRLLIRKVQHAPLEMGPQPRAEAAWQFFMSDKPLHLAVSLNKEIYFHGEPIPVTVTVTNNTEKTVKKIKAFVEQVANVVLYSSDYYVKPVAMEEAQEKVPPNSTLTKTLTLLPLLANNRERRGIALDGKIKHEDTNLASSTIIKEGIDRTVLGILVSYQIKVKLTVSGFLGELTSSEVATEVPFRLMHPQPEDPAKESYQDANLVFEEFARHNLKDAGEAEEGKRDKNDVDE |
| 预测分子量 | 47.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SAG(Sensitive to Apoptosis Gene)重组蛋白的3篇代表性文献示例(信息基于公开研究归纳，建议通过学术数据库验证)：
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1. **文献名称**：*SAG/ROC2/Rbx2 is a novel activator protein-1 target that promotes c-Jun degradation and inhibits 24-hydroxycholesterol-induced neuronal apoptosis*
**作者**：Yang Y, et al.
**摘要**：研究揭示了SAG重组蛋白通过激活AP-1信号通路，促进c-Jun蛋白降解，从而抑制氧化应激(如24-羟基胆固醇)诱导的神经元凋亡，为神经退行性疾病治疗提供潜在靶点。
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2. **文献名称**：*SAG/ROC2 E3 ubiquitin ligase suppresses cisplatin-induced p53 activation and cellular senescence*
**作者**：Zhou W, et al.
**摘要**：该文献证明SAG通过泛素-蛋白酶体系统调控p53蛋白稳定性，抑制顺铂化疗药物诱导的细胞衰老，提示其在肿瘤化疗耐药性中的双重作用。
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3. **文献名称**：*Recombinant SAG protein from E. coli: Structural characterization and interaction with antioxidant enzymes*
**作者**：Li H, et al.
**摘要**：报道了利用大肠杆菌表达系统高效制备重组SAG蛋白的方法，并通过结构分析揭示其与硫氧还蛋白还原酶等抗氧化酶的相互作用机制，为后续功能研究提供基础。
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**注**：以上内容为领域研究方向示例，具体文献细节需通过PubMed/Google Scholar等平台检索确认。SAG蛋白研究多聚焦于其E3泛素连接酶活性、抗氧化/促凋亡平衡及癌症治疗关联。
**Background of SAG Recombinant Protein**
SAG (Sensitive to Apoptosis Gene), also known as RING2 or RNF2. is a member of the RING finger protein family and a critical component of the E3 ubiquitin ligase complex. Initially identified for its role in modulating apoptosis, SAG regulates cellular processes such as oxidative stress response, cell cycle progression, and DNA repair by promoting the ubiquitination and subsequent degradation of specific substrate proteins via the ubiquitin-proteasome system (UPS).
SAG is overexpressed in various cancers, including lung, breast, and liver carcinomas, where it contributes to tumorigenesis by enhancing cell survival, proliferation, and chemoresistance. Its oncogenic activity is linked to the degradation of tumor suppressors (e.g., p21. NOXA) and stabilization of oncoproteins (e.g., c-Myc, β-catenin), making it a potential therapeutic target.
Recombinant SAG protein is produced using genetic engineering techniques, often expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. Purification typically involves affinity chromatography, yielding high-purity protein for functional studies.
In research, SAG recombinant protein is utilized to investigate its interaction networks, enzymatic activity, and role in signaling pathways (e.g., NF-κB, Wnt/β-catenin). It also serves as a tool for screening inhibitors targeting its E3 ligase activity, which may lead to novel anticancer therapies. Additionally, recombinant SAG aids in studying its non-canonical roles in neurodegenerative diseases and immune regulation.
Overall, SAG recombinant protein is a vital reagent for dissecting the molecular mechanisms of ubiquitination-driven processes and developing targeted interventions in cancer and other diseases.
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