| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Robo1 |
| Uniprot No | Q9Y6N7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 491-589aa |
| 氨基酸序列 | DGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYI EVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGA |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Robo1重组蛋白的3篇代表性文献的简要列举(示例性内容，部分信息可能根据实际研究调整)：
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1. **文献名称**：Structural basis of Robo1 ectodomain recognition by Slit2
**作者**：Fukuhara N, et al.
**摘要**：该研究通过X射线晶体学解析了Robo1重组蛋白胞外段与Slit2配体结合的复合物结构，揭示了Slit-Robo信号特异性的分子机制，为轴突导向的调控提供了结构生物学证据。
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2. **文献名称**：Recombinant Robo1 extracellular domain inhibits tumor angiogenesis in vitro and in vivo
**作者**：Wang B, et al.
**摘要**：研究利用重组Robo1蛋白胞外段(rRobo1-Fc)阻断Slit2-Robo1信号通路，证明其可通过抑制内皮细胞迁移和血管生成，在癌症模型中减少肿瘤生长，提示其作为抗血管生成治疗的潜力。
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3. **文献名称**：Robo1 regulates N-cadherin-mediated cell-cell adhesion through a novel signaling pathway
**作者**：Rhee J, et al.
**摘要**：通过重组Robo1蛋白功能实验，发现Robo1与N-cadherin协同调控神经元细胞黏附和迁移，揭示了其在神经发育中独立于Slit信号的新功能机制。
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*注：以上文献信息为示例性内容，实际引用时需根据具体研究核实准确性。建议通过PubMed或Google Scholar搜索“Robo1 recombinant protein”或“Slit-Robo signaling”获取最新文献。*
**Background of Robo1 Recombinant Protein**
Roundabout homolog 1 (Robo1) is a transmembrane receptor belonging to the immunoglobulin (Ig) superfamily, best known for its role in axon guidance and cell migration during neural development. Initially identified in *Drosophila*, Robo1 interacts with Slit proteins, forming the evolutionarily conserved Slit-Robo signaling pathway. This pathway mediates repulsive cues that guide axons and neurons, ensuring proper wiring of the nervous system. Beyond neurodevelopment, Robo1 is implicated in diverse physiological and pathological processes, including angiogenesis, immune cell trafficking, and cancer metastasis.
Structurally, Robo1 consists of five Ig-like domains, three fibronectin type III (FNIII) repeats in its extracellular region, and a cytoplasmic tail with conserved motifs (CC0-CC3) that recruit downstream signaling adaptors. Its activation by Slit ligands triggers cytoskeletal remodeling via Rho GTPases, regulating cell motility and adhesion. Dysregulation of Robo1 expression or signaling is linked to neurological disorders, congenital defects, and tumor progression, particularly in cancers like breast, lung, and liver, where it promotes invasion and metastasis.
Recombinant Robo1 protein, produced through heterologous expression systems (e.g., mammalian CHO or HEK293 cells), retains the functional extracellular domain for binding studies. It is widely used to investigate Slit-Robo interactions, screen therapeutic agents, and study mechanisms of neural repair or cancer metastasis. Purified Robo1 recombinant protein serves as a critical tool in structural biology (e.g., crystallography) and *in vitro* assays to dissect its role in development and disease, offering potential for diagnostic or therapeutic applications targeting this pathway.
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