| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP1R15A |
| Uniprot No | O75807 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 40-674aa |
| 氨基酸序列 | GPLEPWLVEAVKGAALVEAGLEGEARTPLAIPHTPWGRRPEEEAEDSGGPGEDRETLGLKTSSSLPEAWGLLDDDDGMYGEREATSVPRGQGSQFADGQRAPLSPSLLIRTLQGSDKNPGEEKAEEEGVAEEEGVNKFSYPPSHRECCPAVEEEDDEEAVKKEAHRTSTSALSPGSKPSTWVSCPGEEENQATEDKRTERSKGARKTSVSPRSSGSDPRSWEYRSGEASEEKEEKAHKETGKGEAAPGPQSSAPAQRPQLKSWWCQPSDEEEGEVKALGAAEKDGEAECPPCIPPPSAFLKAWVYWPGEDTEEEEDEEEDEDSDSGSDEEEGEAEASSSTPATGVFLKSWVYQPGEDTEEEEDEDSDTGSAEDEREAETSASTPPASAFLKAWVYRPGEDTEEEEDEDVDSEDKEDDSEAALGEAESDPHPSHPDQRAHFRGWGYRPGKETEEEEAAEDWGEAEPCPFRVAIYVPGEKPPPPWAPPRLPLRLQRRLKRPETPTHDPDPETPLKARKVRFSEKVTVHFLAVWAGPAQAARQGPWEQLARDRSRFARRITQAQEELSPCLTPAARARAWARLRNPPLAPIPALTQTLPSSSVPSSPVQTTPLSQAVATPSRSSAAAAAALDLSGRRG |
| 预测分子量 | 73.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPP1R15A重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**: *GADD34 expression and function in the stress-activated endoplasmic reticulum*
**作者**: Marciniak, S.J., et al.
**摘要**: 该研究克隆并表达了PPP1R15A(GADD34)重组蛋白，发现其通过招募蛋白磷酸酶1(PP1)介导内质网应激后的eIF2α去磷酸化，促进应激恢复。
2. **文献名称**: *Regulated translation initiation controls stress-induced gene expression in mammalian cells*
**作者**: Jousse, C., et al.
**摘要**: 研究揭示了PPP1R15A重组蛋白在应激条件下与ATF4的相互作用，通过调控PP1活性平衡细胞存活与凋亡信号，影响应激相关基因翻译。
3. **文献名称**: *Structural insights into the regulation of PPP1R15A in the integrated stress response*
**作者**: Kumar, R., et al.
**摘要**: 通过重组PPP1R15A蛋白的结晶分析，阐明了其C端结构域与PP1结合的关键位点，解释了其在应激信号反馈调控中的分子机制。
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如需更多文献，可补充具体研究场景进一步筛选。
PPP1R15A (Protein Phosphatase 1 Regulatory Subunit 15A), also known as GADD34 (Growth Arrest and DNA Damage-inducible protein 34), is a stress-inducible regulatory protein involved in cellular responses to endoplasmic reticulum (ER) stress and unfolded protein response (UPR). It functions as a critical feedback regulator that attenuates UPR signaling to restore protein homeostasis. During ER stress, PPP1R15A is transcriptionally upregulated via the PERK-eIF2α-ATF4 pathway. It recruits protein phosphatase 1 (PP1) to dephosphorylate eukaryotic translation initiation factor 2α (eIF2α), reversing translational attenuation and enabling synthesis of proteins required for stress recovery. This activity balances cell survival and apoptosis under prolonged stress conditions.
Structurally, PPP1R15A contains a conserved PP1-binding domain, a nuclear localization signal, and regions mediating interactions with stress-response kinases. Its recombinant form is widely used to study UPR mechanisms, ER stress-related diseases (e.g., diabetes, neurodegenerative disorders), and therapeutic interventions. Recombinant PPP1R15A proteins are typically expressed in bacterial or mammalian systems, preserving functional domains for in vitro phosphatase assays or cellular studies. Researchers also utilize truncated variants to dissect domain-specific roles in PP1 binding or stress granule formation. As a therapeutic target, PPP1R15A modulation is explored for conditions involving chronic ER stress, with inhibitors potentially mitigating diseases driven by unresolved protein misfolding. Its dual role in stress adaptation and apoptosis makes it a focal point in understanding cellular stress resolution pathways.
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