| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLRG2 |
| Uniprot No | A4D1S0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-409aa |
| 氨基酸序列 | MEESWEAAPGGQAGAELPMEPVGSLVPTLEQPQVPAKVRQPEGPESSPSPAGAVEKAAGAGLEPSSKKKPPSPRPGSPRVPPLSLGYGVCPEPPSPGPALVKLPRNGEAPGAEPAPSAWAPMELQVDVRVKPVGAAGGSSTPSPRPSTRFLKVPVPESPAFSRHADPAHQLLLRAPSQGGTWGRRSPLAAARTESGCDAEGRASPAEGSAGSPGSPTCCRCKELGLEKEDAALLPRAGLDGDEKLPRAVTLTGLPMYVKSLYWALAFMAVLLAVSGVVIVVLASRAGARCQQCPPGWVLSEEHCYYFSAEAQAWEASQAFCSAYHATLPLLSHTQDFLGRYPVSRHSWVGAWRGPQGWHWIDEAPLPPQLLPEDGEDNLDINCGALEEGTLVAANCSTPRPWVCAKGTQ |
| 预测分子量 | 44.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KLRG2重组蛋白的模拟参考文献示例(请注意，部分文献信息为虚构，仅供格式参考)：
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1. **标题**: *Expression and Functional Characterization of Recombinant KLRG2 in NK Cell Signaling*
**作者**: Zhang Y, et al.
**摘要**: 本研究通过哺乳动物表达系统成功制备了重组KLRG2蛋白，并证实其通过ITIM结构域抑制NK细胞的活化。实验表明，重组KLRG2与配体结合后可招募SHP-1磷酸酶，下调细胞毒性因子的分泌。
2. **标题**: *Structural Insights into KLRG2-Ligand Interaction via Recombinant Protein Crystallography*
**作者**: Tanaka K, et al.
**摘要**: 利用重组KLRG2胞外域蛋白进行晶体结构解析，揭示了其与钙离子依赖性配体E-cadherin的结合模式，为KLRG2在免疫监视中的作用机制提供了结构基础。
3. **标题**: *KLRG2 Recombinant Protein Modulates T Cell Exhaustion in Chronic Viral Infection*
**作者**: Müller R, et al.
**摘要**: 通过体外重组KLRG2蛋白实验，发现其与CD8+ T细胞表面的结合可增强PD-1信号通路，促进T细胞耗竭表型，提示KLRG2在慢性感染中的潜在治疗靶点。
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**注**：以上文献为示例性内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实发表的论文。若需具体文献，建议结合最新研究进展进一步查询。
**Background of KLRG2 Recombinant Protein**
KLRG2 (Killer Cell Lectin-Like Receptor G2) is a transmembrane protein belonging to the C-type lectin-like receptor family, primarily expressed on natural killer (NK) cells and subsets of T cells. It functions as an inhibitory receptor, modulating immune cell activity by transmitting intracellular signals through its cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). KLRG2 interacts with specific ligands, such as E-cadherin, to regulate immune responses, particularly in maintaining peripheral tolerance and preventing excessive immune activation.
Recombinant KLRG2 protein is engineered in vitro using expression systems like mammalian or insect cells to mimic its native structure and function. This protein typically includes the extracellular domain responsible for ligand binding, which depends on calcium-dependent carbohydrate recognition. Researchers utilize KLRG2 recombinant protein to study its role in immune regulation, including its impact on NK cell cytotoxicity, T cell exhaustion, and immune evasion mechanisms in diseases like cancer or chronic infections.
Studies suggest KLRG2 may serve as a checkpoint molecule, influencing adaptive immune responses in tumor microenvironments. Its upregulation in exhausted T cells has linked it to impaired anti-tumor immunity, making it a potential therapeutic target. Additionally, recombinant KLRG2 is used in ligand-rebinding assays, structural studies, and antibody development to explore its interaction networks and signaling pathways.
Overall, KLRG2 recombinant protein serves as a critical tool for dissecting immune inhibitory pathways and developing strategies to modulate immune responses in immunotherapy and autoimmune disease research.
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