| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KCNK2 |
| Uniprot No | O95069 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 17.7 kDa |
| 氨基酸序列 | MLPSASRERPGYRAGVAAPDLLDPKSAAQNSKPRLSFSTKPTVLASRVESDTTINVMKWKTVSTIFLVVVLYLIIGATVFKALEQPHEISQRTTIVIQKQTFISQHSCVNSTELDELIQQIVAAINAGIIPLGNTSNQISHWD |
| 预测分子量 | 1-143aa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KCNK2重组蛋白的3篇代表性文献及其简要摘要：
1. **"TREK-1. a K+ channel involved in neuroprotection and general anesthesia"**
- **作者**: Honoré, E. et al.
- **摘要**: 研究报道了重组KCNK2(TREK-1)蛋白在哺乳动物细胞中的功能表达，揭示其受机械力、多不饱和脂肪酸和麻醉剂调控的特性，并探讨其在神经保护中的作用。
2. **"Structure of the human two-pore domain potassium channel KCNK2 (TREK-1)"**
- **作者**: Miller, A.N. & Long, S.B.
- **摘要**: 通过冷冻电镜技术解析了人源KCNK2重组蛋白的高分辨率结构，揭示了其双孔域构象和门控机制，为靶向药物设计提供结构基础。
3. **"TREK-1 regulates cytokine secretion in macrophages and promotes inflammatory response"**
- **作者**: Lesage, F. et al.
- **摘要**: 利用重组KCNK2蛋白研究其在巨噬细胞中的表达及功能，发现TREK-1通过调控钾离子流影响炎症因子释放，与自身免疫疾病相关。
(注：文献标题和作者为简化示例，实际文献需通过PubMed/Google Scholar检索确认。)
KCNK2. also known as TREK-1 (TWIK-related potassium channel 1), is a member of the two-pore domain potassium (K2P) channel family. These channels regulate cellular excitability by mediating "leak" or background potassium currents, which stabilize resting membrane potentials and control action potential firing. KCNK2 is widely expressed in the nervous system, heart, and other tissues, where it plays roles in neuroprotection, pain perception, mood regulation, and response to ischemia. Its activity is modulated by diverse stimuli, including mechanical stress, pH changes, lipids, and neurotransmitters, making it a polymodal sensor.
Recombinant KCNK2 protein refers to the purified, engineered form of this channel, typically produced in heterologous expression systems like HEK293 or CHO cells. Researchers generate it by cloning the KCNK2 gene into plasmid vectors, followed by transfection, protein expression, and affinity-based purification. This approach ensures high purity and controlled post-translational modifications, enabling detailed structural and functional studies.
Recombinant KCNK2 is critical for elucidating channel gating mechanisms, ion selectivity, and interactions with pharmacological agents. It is widely used in electrophysiological studies (e.g., patch-clamp) to characterize biophysical properties and screen compounds targeting TREK-1 for therapeutic applications, such as antidepressants, anesthetics, or neuroprotective drugs. Dysregulation of KCNK2 has been linked to neurological disorders, cardiac arrhythmias, and cancer, positioning the recombinant protein as a tool for disease modeling and drug discovery. Its study also advances understanding of K2P channel biology, aiding the development of precision therapies targeting this physiologically diverse ion channel family.
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