| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HSD17B4 |
| Uniprot No | P51659 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-736aa |
| 氨基酸序列 | MGSPLRFDGRVVLVTGAGAGLGRAYALAFAERGALVVVNDLGGDFKGVGKGSLAADKVVEEIRRRGGKAVANYDSVEEGEKVVKTALDAFGRIDVVVNNAGILRDRSFARISDEDWDIIHRVHLRGSFQVTRAAWEHMKKQKYGRIIMTSSASGIYGNFGQANYSAAKLGLLGLANSLAIEGRKSNIHCNTIAPNAGSRMTQTVMPEDLVEALKPEYVAPLVLWLCHESCEENGGLFEVGAGWIGKLRWERTLGAIVRQKNHPMTPEAVKANWKKICDFENASKPQSIQESTGSIIEVLSKIDSEGGVSANHTSRATSTATSGFAGAIGQKLPPFSYAYTELEAIMYALGVGASIKDPKDLKFIYEGSSDFSCLPTFGVIIGQKSMMGGGLAEIPGLSINFAKVLHGEQYLELYKPLPRAGKLKCEAVVADVLDKGSGVVIIMDVYSYSEKELICHNQFSLFLVGSGGFGGKRTSDKVKVAVAIPNRPPDAVLTDTTSLNQAALYRLSGDWNPLHIDPNFASLAGFDKPILHGLCTFGFSARRVLQQFADNDVSRFKAIKARFAKPVYPGQTLQTEMWKEGNRIHFQTKVQETGDIVISNAYVDLAPTSGTSAKTPSEGGKLQSTFVFEEIGRRLKDIGPEVVKKVNAVFEWHITKGGNIGAKWTIDLKSGSGKVYQGPAKGAADTTIILSDEDFMEVVLGKLDPQKAFFSGRLKARGNIMLSQKLQMILKDYAKL |
| 预测分子量 | 86.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HSD17B4重组蛋白的3篇参考文献示例(注：文献信息为示例性概括，实际引用需核对真实论文)：
---
1. **文献名称**: *"Cloning and functional characterization of human 17β-hydroxysteroid dehydrogenase type 4 in Escherichia coli"*
**作者**: Smith A, et al.
**摘要**: 该研究报道了人源HSD17B4基因在大肠杆菌中的重组表达及纯化，验证了其双功能酶活性(同时具有脱氢酶和酰基辅酶A水解酶活性)，并探讨了其在类固醇和脂肪酸代谢中的作用。
2. **文献名称**: *"Structural insights into the bifunctionality of HSD17B4 through recombinant protein crystallography"*
**作者**: Lee J, et al.
**摘要**: 通过重组HSD17B4蛋白的晶体结构解析，揭示了其N端脱氢酶结构域与C端水解酶结构域的协同机制，为理解HSD17B4在过氧化物酶体中的代谢功能提供了分子基础。
3. **文献名称**: *"Recombinant HSD17B4 deficiency model reveals its role in peroxisomal β-oxidation disorders"*
**作者**: Müller C, et al.
**摘要**: 利用重组HSD17B4蛋白构建体外酶活性检测体系，发现其功能缺失导致支链脂肪酸代谢异常，与遗传性疾病(如D-双功能蛋白缺陷症)的病理机制直接相关。
---
如需具体文献，建议通过PubMed或Google Scholar搜索关键词“HSD17B4 recombinant protein”获取最新研究。
HSD17B4. also known as 17β-hydroxysteroid dehydrogenase type 4 or peroxisomal multifunctional enzyme type 2 (MFE-2), is a key protein involved in lipid metabolism and steroid hormone regulation. It is encoded by the *HSD17B4* gene in humans and plays dual enzymatic roles: as a 17β-hydroxysteroid dehydrogenase, it participates in the interconversion of active and inactive forms of steroid hormones (e.g., converting estrone to estradiol), and as a D-3-hydroxyacyl-CoA dehydrogenase, it contributes to the peroxisomal β-oxidation of branched-chain fatty acids and bile acid precursors. This multifunctionality links HSD17B4 to critical metabolic pathways, particularly in the liver, brain, and reproductive tissues.
Mutations in *HSD17B4* are associated with severe genetic disorders, such as Zellweger syndrome spectrum (a peroxisome biogenesis disorder) and adult-onset type II peroxisomal bifunctional enzyme deficiency. These conditions manifest with neurological dysfunction, liver abnormalities, and disrupted steroid homeostasis. Recombinant HSD17B4 protein, typically produced in bacterial (e.g., *E. coli*) or mammalian expression systems, retains enzymatic activities for research and therapeutic applications. Its production enables functional studies to dissect substrate specificity, catalytic mechanisms, and interactions with metabolic partners. Purification methods often involve affinity tags (e.g., His-tag) followed by chromatographic refinement.
Research on recombinant HSD17B4 aids in understanding lipid-related diseases, hormone-dependent cancers, and peroxisomal disorders. It also serves as a tool for drug screening targeting metabolic syndromes or enzyme deficiencies. Recent studies explore its role in cellular redox balance and potential crosstalk with mitochondrial pathways, highlighting its broader impact on cellular metabolism.
×
