| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR75 |
| Uniprot No | O95800 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-540aa |
| 氨基酸序列 | MNSTGHLQDAPNATSLHVPHSQEGNSTSLQEGLQDLIHTATLVTCTFLLAVIFCLGSYGNFIVFLSFFDPAFRKFRTNFDFMILNLSFCDLFICGVTAPMFTFVLFFSSASSIPDAFCFTFHLTSSGFIIMSLKTVAVIALHRLRMVLGKQPNRTASFPCTVLLTLLLWATSFTLATLATLKTSKSHLCLPMSSLIAGKGKAILSLYVVDFTFCVAVVSVSYIMIAQTLRKNAQVRKCPPVITVDASRPQPFMGVPVQGGGDPIQCAMPALYRNQNYNKLQHVQTRGYTKSPNQLVTPAASRLQLVSAINLSTAKDSKAVVTCVIIVLSVLVCCLPLGISLVQVVLSSNGSFILYQFELFGFTLIFFKSGLNPFIYSRNSAGLRRKVLWCLQYIGLGFFCCKQKTRLRAMGKGNLEVNRNKSSHHETNSAYMLSPKPQKKFVDQACGPSHSKESMVSPKISAGHQHCGQSSSTPINTRIEPYYSIYNSSPSQEESSPCNLQPVNSFGFANSYIAMHYHTTNDLVQEYDSTSAKQIPVPSV |
| 预测分子量 | 60.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GPR75重组蛋白的3篇代表性文献(信息基于公开研究整理，部分为虚拟示例)：
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1. **文献名称**：*Structural insights into GPR75 activation and ligand binding through recombinant protein expression*
**作者**：Smith, J. et al.
**摘要**：本研究通过昆虫细胞系统表达并纯化GPR75重组蛋白，结合冷冻电镜技术解析其三维结构，揭示了其与天然配体(如20-HETE)结合的分子机制，为靶向GPR75的药物设计提供结构基础。
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2. **文献名称**：*GPR75 knockdown ameliorates obesity-related metabolic dysfunction via recombinant receptor studies*
**作者**：Chen, L. et al.
**摘要**：利用重组GPR75蛋白模型，研究发现该受体在脂肪细胞能量代谢中起关键作用。基因敲除或药理抑制GPR75可显著改善高脂饮食小鼠的胰岛素抵抗和体重增长，提示其作为肥胖治疗靶点的潜力。
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3. **文献名称**：*Development of a high-throughput screening assay for GPR75 agonists using recombinant HEK293 cells*
**作者**：Kim, S. et al.
**摘要**：通过在HEK293细胞中稳定表达GPR75重组蛋白，建立了基于钙流检测的激动剂高通量筛选平台，并鉴定出多个小分子候选化合物，为研究GPR75信号通路及疾病干预提供工具。
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注：以上文献为示例性质，实际研究中建议通过PubMed或Web of Science以“GPR75 recombinant”为关键词检索最新论文。
GPR75 (G Protein-Coupled Receptor 75) is an orphan receptor belonging to the class A G protein-coupled receptor (GPCR) family. Initially identified through genomic sequencing, its endogenous ligand remains unidentified, though it is speculated to interact with neuropeptides or lipid-derived molecules. GPR75 has garnered significant attention due to its potential role in metabolic regulation and energy homeostasis. Genome-wide association studies (GWAS) have linked GPR75 gene variants to obesity and body mass index (BMI) variations, with loss-of-function mutations associated with reduced obesity risk. This positions GPR75 as a promising therapeutic target for metabolic disorders.
Recombinant GPR75 protein is typically produced using heterologous expression systems such as HEK293 or insect cells, enabling large-scale purification for functional studies. The protein features seven transmembrane α-helices, extracellular N-terminal domains, and intracellular loops critical for G protein coupling. Researchers often engineer epitope tags (e.g., His-tag, FLAG-tag) to facilitate detection and purification. Its recombinant form is essential for structural studies (e.g., cryo-EM), ligand-binding assays, and signaling pathway characterization.
Current applications include screening synthetic ligands or antibodies, investigating downstream signaling cascades (potentially involving Gαq/11 or β-arrestin pathways), and validating CRISPR-edited cellular models. Challenges persist in confirming its native ligand and tissue-specific signaling mechanisms. Nevertheless, GPR75 recombinant protein serves as a vital tool for decoding its physiological roles in adipogenesis, insulin sensitivity, and neuroendocrine functions, bridging genetic discoveries to mechanistic insights in metabolic disease research.
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