| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | ACB1 |
| Uniprot No | P31787 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-87aa |
| 氨基酸序列 | MVSQLFEEKAKAVNELPTKPSTDELLELYALYKQATVGDNDKEKPGIFNMKDRYKWEAWENLKGKSQEDAEKEYIALVDQLIAKYSS |
| 预测分子量 | 12.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACB1重组蛋白的模拟参考文献示例(内容为假设性描述，仅供参考)：
---
1. **标题**: *Heterologous Expression and Functional Characterization of Recombinant ACB1 in Saccharomyces cerevisiae*
**作者**: Smith J, Brown K, Lee M
**摘要**: 本研究通过在大肠杆菌中表达ACB1重组蛋白，优化了其可溶性表达条件，并利用亲和层析技术实现了高效纯化。实验表明，重组ACB1能够特异性结合长链脂肪酸，验证了其在脂质转运中的潜在作用。
2. **标题**: *Structural Insights into ACB1 Recombinant Protein by Cryo-EM Analysis*
**作者**: Zhang Y, Wang L, Chen X
**摘要**: 通过冷冻电镜技术解析了ACB1重组蛋白的三维结构，揭示了其酰基-CoA结合域的关键氨基酸残基。该结构为研究ACB1在细胞代谢中的分子机制提供了重要依据。
3. **标题**: *ACB1 Recombinant Protein Enhances β-Oxidation in Hepatocyte Models*
**作者**: Gupta R, Patel S, Kim H
**摘要**: 利用肝细胞模型证明，外源添加ACB1重组蛋白可显著增强线粒体β-氧化活性，提示其在调节能量代谢和缓解脂毒性中的治疗潜力。
4. **标题**: *Development of an ACB1-Specific Monoclonal Antibody Using Recombinant Protein as Antigen*
**作者**: Müller T, Schmidt F, Wagner E
**摘要**: 以纯化的ACB1重组蛋白为抗原，成功制备了高特异性和亲和力的单克隆抗体，为ACB1的免疫检测及疾病诊断工具开发奠定了基础。
---
**注**：以上文献为模拟内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。若需进一步协助，建议提供更具体的蛋白背景或研究领域。
**Background of ACB1 Recombinant Protein**
ACB1 (Acyl-CoA-binding protein 1), also known as diazepam-binding inhibitor (DBI), is a highly conserved, multifunctional protein involved in lipid metabolism, cellular signaling, and stress responses. It binds medium- and long-chain acyl-CoA esters with high affinity, acting as an intracellular carrier to regulate fatty acid synthesis, β-oxidation, and lipid transport. ACB1 is ubiquitously expressed across tissues, with notable roles in the brain, liver, and endocrine systems, where it modulates neurosteroid synthesis and mitochondrial function.
The recombinant ACB1 protein is produced using genetic engineering techniques, often in *E. coli* or mammalian expression systems, to ensure high purity and bioactivity. Recombinant ACB1 retains its native structure, enabling studies on its molecular interactions, enzymatic regulation, and lipid-binding kinetics. Its applications span *in vitro* assays, structural biology (e.g., crystallography), and drug discovery, particularly targeting metabolic disorders, neurodegenerative diseases, and cancers linked to dysregulated lipid metabolism.
Recent research highlights ACB1's role in cellular stress adaptation, including autophagy and apoptosis, and its potential as a biomarker or therapeutic target. Pathologically, altered ACB1 expression is associated with obesity, diabetes, and neurological conditions. Recombinant ACB1 facilitates mechanistic insights into these processes, offering a tool to explore therapeutic interventions. Continued studies aim to unravel its pleiotropic functions and exploit its regulatory pathways for biomedical applications.
(Word count: 199)
×
