| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CTPS1 |
| Uniprot No | P17812 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-591aa |
| 氨基酸序列 | MKYILVTGGVISGIGKGIIASSVGTILKSCGLHVTSIKIDPYINIDAGTFSPYEHGEVFVLDDGGEVDLDLGNYERFLDIRLTKDNNLTTGKIYQYVINKERKGDYLGKTVQVVPHITDAIQEWVMRQALIPVDEDGLEPQVCVIELGGTVGDIESMPFIEAFRQFQFKVKRENFCNIHVSLVPQPSSTGEQKTKPTQNSVRELRGLGLSPDLVVCRCSNPLDTSVKEKISMFCHVEPEQVICVHDVSSIYRVPLLLEEQGVVDYFLRRLDLPIERQPRKMLMKWKEMADRYDRLLETCSIALVGKYTKFSDSYASVIKALEHSALAINHKLEIKYIDSADLEPITSQEEPVRYHEAWQKLCSAHGVLVPGGFGVRGTEGKIQAIAWARNQKKPFLGVCLGMQLAVVEFSRNVLGWQDANSTEFDPTTSHPVVVDMPEHNPGQMGGTMRLGKRRTLFQTKNSVMRKLYGDADYLEERHRHRFEVNPVWKKCLEEQGLKFVGQDVEGERMEIVELEDHPFFVGVQYHPEFLSRPIKPSPPYFGLLLASVGRLSHYLQKGCRLSPRDTYSDRSGSSSPDSEITELKFPSINHD |
| 预测分子量 | 68.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CTPS1重组蛋白的3篇参考文献的简要整理(注：文献为虚拟示例，仅供参考)：
1. **文献名称**: *"Recombinant human CTPS1: expression, purification, and enzymatic characterization"*
**作者**: Smith A, et al.
**摘要**: 报道了人源CTPS1在大肠杆菌中的重组表达和镍柱纯化方法，分析了其酶动力学参数及对底物UTP/ATP的依赖性。
2. **文献名称**: *"Structural insights into CTPS1 filament formation by cryo-EM"*
**作者**: Chen L, et al.
**摘要**: 通过冷冻电镜解析了重组CTPS1蛋白在体外自组装形成的丝状结构，揭示了其聚合机制与酶活性调控的分子基础。
3. **文献名称**: *"Functional analysis of CTPS1 mutations in immunodeficiency disorders"*
**作者**: Wang Y, et al.
**摘要**: 利用重组CTPS1蛋白系统验证了多个临床突变体(如E151K)对酶活性和负反馈调节的影响，为免疫缺陷疾病机制提供依据。
(提示：实际文献需通过PubMed/Google Scholar以“CTPS1 recombinant protein”等关键词检索，推荐结合UniProt数据库ID P17812获取相关研究)
CTP synthase 1 (CTPS1) is a critical enzyme in the nucleotide biosynthesis pathway, responsible for catalyzing the conversion of uridine triphosphate (UTP) to cytidine triphosphate (CTP), the final step in *de novo* pyrimidine nucleotide synthesis. This ATP-dependent reaction involves the amination of UTP to form CTP, a precursor essential for DNA/RNA synthesis, lipid metabolism, and cellular signaling. CTPS1 is ubiquitously expressed in proliferating cells, particularly in tissues with high metabolic demands, such as immune cells, cancer cells, and rapidly dividing stem cells. Its activity is tightly regulated by feedback mechanisms, substrate availability, and post-translational modifications to maintain nucleotide pool balance.
Recombinant CTPS1 protein is produced via heterologous expression systems (e.g., *E. coli*, yeast, or mammalian cells*) to study its structure, enzymatic kinetics, and regulatory mechanisms. Structural studies reveal that CTPS1 forms dynamic filamentous structures (cytoophidia or metabolic filaments) under certain conditions, which modulate its enzymatic activity and cellular localization. These filaments are thought to act as storage compartments or allosteric regulators, linking metabolic state to enzyme function.
Research on recombinant CTPS1 has implications in cancer therapy, antiviral drug development, and immune modulation. Inhibitors targeting CTPS1 are explored for their potential to block nucleotide synthesis in hyperproliferative diseases, such as leukemia or solid tumors, or to combat viruses reliant on host nucleotide pools. Additionally, CTPS1 dysfunction is linked to rare genetic disorders affecting immunity and neurodevelopment. The recombinant protein serves as a vital tool for biochemical assays, high-throughput screening, and elucidating the role of nucleotide metabolism in health and disease.
×
