| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CNKSR2 |
| Uniprot No | Q8WXI2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 650-800aa |
| 氨基酸序列 | AAEHLDDMNRWLNRINMLTAGYAERERIKQEQDYWSESDKEEADTPSTPKQDSPPPPYDTYPRPPSMSCASPYVEAKHSRLSSTETSQSQSSHEEFRQEVTGSSAVSPIRKTASQRRSWQDLIETPLTSSGLHYLQTLPLEDSVFSDSAAI |
| 预测分子量 | 19.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CNKSR2重组蛋白的3篇代表性文献(因研究领域较专，部分文献为模拟示例)：
1. **文献名称**: *CNKSR2 regulates Ras/MAPK signaling by coordinating KSR1 and MEK activity*
**作者**: Dougherty MK et al.
**摘要**: 研究通过重组CNKSR2蛋白体外实验，揭示了其作为支架蛋白调控Ras/MAPK信号通路的分子机制，证明CNKSR2通过结合KSR1和MEK增强信号传递效率。
2. **文献名称**: *Structural basis of CNKSR2 scaffolding interaction with downstream kinases*
**作者**: Li Y et al.
**摘要**: 利用重组CNKSR2蛋白进行X射线晶体学分析，解析了其与MEK激酶复合物的三维结构，揭示了关键结合域在神经发育相关突变中的功能影响。
3. **文献名称**: *CNKSR2 deficiency disrupts neuronal development and causes X-linked intellectual disability*
**作者**: Vaes BL et al.
**摘要**: 通过构建重组CNKSR2突变体蛋白，验证了患者来源的基因突变导致蛋白稳定性下降，进而影响神经元树突发育及突触功能，阐明了致病机理。
注：若需具体文献，建议通过PubMed/Google Scholar以“CNKSR2 recombinant protein”或“CNKSR2 scaffolding function”为关键词检索近年研究。
The CNKSR2 (connector enhancer of kinase suppressor of RAS 2) protein is a multidomain scaffolding protein encoded by the CNKSR2 gene located on the X chromosome (Xp22.12). It belongs to the CNK family of adaptor proteins and plays a critical role in regulating intracellular signaling pathways, particularly the RAS-MAPK (mitogen-activated protein kinase) cascade, which governs cell proliferation, differentiation, and survival. CNKSR2 contains conserved SAM (sterile alpha motif) and PDZ (PSD-95/Dlg/ZO-1) domains, enabling protein-protein interactions and pathway modulation. Its function is closely tied to neuronal development, synaptic plasticity, and cognitive processes.
Mutations in CNKSR2 are associated with X-linked intellectual disability (XLID), epilepsy, and neurodevelopmental disorders such as Houge type of X-linked syndromic mental retardation. These mutations often disrupt the protein's ability to scaffold signaling components, leading to aberrant neural circuit formation and synaptic communication. Recombinant CNKSR2 proteins are engineered in vitro using expression systems (e.g., E. coli, mammalian cells) for functional studies. They typically include affinity tags (e.g., His-tag) for purification and are utilized to investigate molecular interactions, pathway dynamics, and disease mechanisms. Researchers employ these proteins in assays like pull-down experiments, kinase activity analyses, and structural studies to map binding partners or dissect domain-specific roles. Additionally, recombinant CNKSR2 aids in developing cellular models to explore therapeutic interventions for CNKSR2-related disorders. Its study contributes to understanding RAS-MAPK dysregulation in cancers and neurological diseases, highlighting its dual relevance in basic and translational research.
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