| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD48 |
| Uniprot No | P09326 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-220aa |
| 氨基酸序列 | QGHLVHMTVVSGSNVTLNISESLPENYKQLTWFYTFDQKIVEWDSRKSKY FESKFKGRVR LDPQSGALYISKVQKEDNSTYIMRVLKKTGNEQEWKIK LQVLDPVPKPVIKIEKIEDMDD NCYLKLSCVIPGESVNYTWYGDKRPF PKELQNSVLETTLMPHNYSRCYTCQVSNSVSSKN GTVCLSPPCTLARS |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD48重组蛋白的3篇参考文献的简要信息(基于模拟生成，非真实文献)：
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1. **标题**：*Structural and Functional Characterization of Recombinant CD48 in Immune Synapse Formation*
**作者**：Smith A, et al.
**摘要**：本研究通过哺乳动物表达系统成功制备了重组CD48蛋白，并利用表面等离子共振技术揭示了其与CD2的亲和力。研究证实CD48在T细胞与抗原呈递细胞的免疫突触形成中起关键作用，为靶向免疫调节提供了理论基础。
2. **标题**：*Recombinant CD48 Binds to Mycobacterium tuberculosis Protein LpqH and Modulates Innate Immunity*
**作者**：Chen L, et al.
**摘要**：作者通过原核表达系统纯化了功能性CD48重组蛋白，发现其与结核分枝杆菌蛋白LpqH特异性结合，并激活巨噬细胞的NF-κB信号通路。该研究提示CD48可能在宿主抗细菌免疫中具有双重调节功能。
3. **标题**：*CD48 Recombinant Protein Enhances NK Cell Cytotoxicity via Interaction with CD244*
**作者**：Tanaka K, et al.
**摘要**：通过昆虫细胞表达系统获得高纯度CD48重组蛋白，实验表明其与NK细胞表面CD244结合后显著增强细胞毒性颗粒的释放，为癌症免疫治疗中NK细胞功能调控提供了潜在策略。
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**说明**：以上文献为模拟生成，实际引用时需通过学术数据库(如PubMed、Web of Science)核实真实论文。建议结合关键词“CD48 recombinant protein”及“immune function”进一步检索近年研究。
CD48. also known as Signaling Lymphocytic Activation Molecule Family member 2 (SLAMF2) or B-cell Membrane protein 1 (BCM1), is a cell surface glycoprotein belonging to the immunoglobulin (Ig) superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored protein primarily expressed on hematopoietic cells, including lymphocytes, macrophages, dendritic cells, and platelets. CD48 functions as a ligand for CD244 (SLAMF4) and CD2 receptors, playing a critical role in modulating immune cell interactions, particularly in T-cell and natural killer (NK) cell activation, adhesion, and signaling. It participates in both innate and adaptive immune responses by facilitating cell-cell communication during infections, inflammation, and immune surveillance.
The CD48 protein structure comprises two extracellular Ig-like domains (V and C2-type), a short stalk region, and a GPI anchor for membrane attachment. Its interaction with CD244 (expressed on NK cells and cytotoxic T cells) regulates cytotoxicity and cytokine production, while binding to CD2 (on T cells) enhances T-cell activation. CD48 also serves as a receptor for bacterial components, such as the fimbrial protein FimH from *E. coli*, and is exploited by pathogens like *Mycoplasma pneumoniae* and HIV (via gp120) for host cell adhesion or immune evasion.
Recombinant CD48 protein is produced using expression systems (e.g., mammalian, insect, or bacterial cells) to study its biological functions, structural features, and ligand interactions. It is widely used in immunological research, including assays to investigate T/NK cell activation pathways, host-pathogen interactions, and therapeutic target discovery. Purified CD48 also aids in developing diagnostic tools or inhibitors against infections and immune-related disorders. Its recombinant form retains functional epitopes, enabling applications in antibody development, protein-protein interaction studies, and vaccine research.
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