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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCNJL |
| Uniprot No | Q8IV13 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 315-435aa |
| 氨基酸序列 | MVPGTPPTPTQVLFQPPAYPALGQPATTLAQFQTPVQDLCLAYRDSLQAHRSGSLLSGSTGSSLHTPYQPLQPLDMCPVPVPASLSMHMAIAAEPRHCLATTYGSSYFSGSHMFPTGCFDR |
| 预测分子量 | 14.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCNJL重组蛋白的参考文献示例(注：以下为假设性文献，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *"Functional characterization of recombinant CCNJL protein in osteoblast differentiation"*
**作者**: Smith A, et al.
**摘要**: 本研究通过哺乳动物细胞表达系统成功制备重组CCNJL蛋白，并发现其通过激活BMP/Smad信号通路显著促进成骨细胞分化，为骨再生治疗提供潜在靶点。
2. **文献名称**: *"Expression and purification of CCNJL in E. coli: Implications for structural studies"*
**作者**: Tanaka K, et al.
**摘要**: 报道了一种基于大肠杆菌的高效重组CCNJL蛋白表达与纯化策略，通过优化诱导条件获得可溶性蛋白，为后续的X射线晶体学结构解析奠定基础。
3. **文献名称**: *"CCNJL modulates angiogenesis via interaction with VEGF receptor-2"*
**作者**: Gupta R, et al.
**摘要**: 利用重组CCNJL蛋白进行体外实验，揭示其通过与VEGFR2结合增强血管内皮细胞迁移和管腔形成，提示其在缺血性疾病中的治疗潜力。
4. **文献名称**: *"Proteomic analysis of CCNJL-binding partners in tumor microenvironment"*
**作者**: Li X, et al.
**摘要**: 通过重组CCNJL亲和层析结合质谱技术，鉴定出其在肿瘤微环境中的新型相互作用蛋白(如整合素α5β1)，阐明其调控肿瘤侵袭的分子机制。
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**注意**：以上文献为示例性内容，实际研究中建议通过PubMed、Web of Science等平台检索关键词(如“CCNJL recombinant protein”或“CCNJL expression”)获取真实文献。若研究领域较新，可扩展至CCN蛋白家族(如CTGF、NOV)相关研究作为参考。
CCNJL (Cellular Communication Network factor Jelly-Like) is a member of the CCN family of matricellular proteins, which play critical roles in regulating cell-cell communication, adhesion, proliferation, and differentiation. The CCN family comprises six conserved members (CCN1-6), and CCNJL shares structural homology with these proteins but exhibits distinct features in domain organization and functional regulation. Structurally, CCNJL typically contains four conserved modular domains: an insulin-like growth factor-binding protein (IGFBP) domain, a von Willebrand factor type C (VWC) domain, a thrombospondin type 1 (TSP1) domain, and a C-terminal cysteine-knot (CT) domain. These domains enable interactions with extracellular matrix components, growth factors, and cell-surface receptors, modulating signaling pathways such as TGF-β, Wnt, and integrin-mediated cascades.
Functionally, CCNJL is implicated in tissue repair, angiogenesis, and embryonic development. It regulates cellular responses to injury or stress by balancing pro-survival and pro-apoptotic signals. Studies suggest its involvement in cancer progression, where it may act as a tumor suppressor or promoter depending on context. For instance, CCNJL overexpression has been linked to suppressed proliferation in certain cancers, while its downregulation correlates with metastasis and drug resistance.
Recombinant CCNJL proteins are engineered using expression systems like *E. coli* or mammalian cells, often tagged for purification and detection. These proteins serve as tools to study CCNJL's interactions and mechanisms in vitro and in vivo. Despite progress, its full biological significance remains under investigation, particularly its role in fibrosis, inflammation, and cross-talk with other CCN proteins. Understanding CCNJL's context-dependent functions could unlock therapeutic potential for diseases involving dysregulated extracellular matrix dynamics.
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