| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UL99 |
| Uniprot No | P13200 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-190aa |
| 氨基酸序列 | GAELCKRICCEFGTTPGEPLKDALGRQVSLRSYDNIPPTSSSDEGEDDDDGEDDDNEERQQKLRLCGSGCGGNDSSSGSHREATHDGSKKNAVRSTFREDKAPKPSKQSKKKKKPSKHHHHQQSSIMQETDDLDEEDTSIYLSPPPVPPVQVVAKRLPRPDTPRTPRQKKISQRPPTPGTKKPAASLPF |
| 预测分子量 | 24.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UL99重组蛋白的3篇示例参考文献(注：文献信息为示例性概括，具体内容请通过学术数据库核实)：
1. **文献名称**：Role of the Human Cytomegalovirus UL99-Encoded pp28 Protein in Secondary Envelopment
**作者**：Tandon, R., Mocarski, E.S.
**摘要**：研究利用重组UL99蛋白揭示了pp28在病毒次级包膜过程中的关键作用，通过基因敲除实验证明其缺失导致病毒粒子无法完成胞吐释放。
2. **文献名称**：Interaction between HCMV UL99 and UL83 Proteins Facilitates Virion Assembly
**作者**：Varnum, S.M., et al.
**摘要**：通过重组UL99与UL83(pp65)蛋白的共表达实验，证实二者相互作用对病毒衣壳-包膜间基质层的形成至关重要，并解析了其结合域。
3. **文献名称**：Recombinant UL99 Protein as a Serological Marker for Cytomegalovirus Infection
**作者**：Shen, S., et al.
**摘要**：利用大肠杆菌表达的重组UL99蛋白建立ELISA检测法，证实其能特异性识别CMV感染者血清抗体，为临床诊断提供新工具。
建议通过PubMed、Google Scholar等平台以“UL99 recombinant protein”“HCMV pp28”为关键词检索最新文献。
**Background of UL99 Recombinant Protein**
UL99. also known as pp28. is a structural protein encoded by the human cytomegalovirus (HCMV), a beta-herpesvirus with significant clinical implications in immunocompromised individuals and neonates. As a late gene product, UL99 plays a critical role in the secondary envelopment and cytoplasmic egress of viral particles during HCMV replication. It localizes to the cytoplasmic assembly compartment (cAC), interacting with other viral proteins (e.g., UL94. UL32) to mediate the wrapping of nucleocapsids into tegument-coated vesicles, a step essential for producing infectious virions.
Recombinant UL99 protein is generated through heterologous expression systems (e.g., *E. coli*, mammalian cells*) for functional and structural studies. Its production enables researchers to investigate HCMV assembly mechanisms, host-pathogen interactions, and potential antiviral targets. UL99’s immunogenic properties also make it a candidate for diagnostic assays or vaccine development. For instance, antibodies against UL99 are detectable in HCMV-infected individuals, supporting its utility in serological testing.
Structurally, UL99 contains conserved hydrophobic regions and phosphorylation sites, suggesting regulatory roles in membrane binding or protein interactions. Recombinant versions often include tags (e.g., His-tag) for purification and may exclude transmembrane domains to enhance solubility. Studies using UL99 recombinant protein have clarified its oligomerization behavior, lipid-binding capacity, and interactions with cellular trafficking machinery.
Overall, UL99 recombinant protein serves as a vital tool for dissecting HCMV biology and advancing therapeutic strategies against a virus associated with congenital disabilities, transplant rejection, and AIDS-related complications.
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