| 纯度 | >90%SDS-PAGE. |
| 种属 | E. coli |
| 靶点 | recQ |
| Uniprot No | P15043 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-609aa |
| 氨基酸序列 | MAQAEVLNLE SGAKQVLQET FGYQQFRPGQ EEIIDTVLSG RDCLVVMPTG GGKSLCYQIP ALLLNGLTVV VSPLISLMKD QVDQLQANGV AAACLNSTQT REQQLEVMTG CRTGQIRLLY IAPERLMLDN FLEHLAHWNP VLLAVDEAHC ISQWGHDFRP EYAALGQLRQ RFPTLPFMAL TATADDTTRQ DIVRLLGLND PLIQISSFDR PNIRYMLMEK FKPLDQLMRY VQEQRGKSGI IYCNSRAKVE DTAARLQSKG ISAAAYHAGL ENNVRADVQE KFQRDDLQIV VATVAFGMGI NKPNVRFVVH FDIPRNIESY YQETGRAGRD GLPAEAMLFY DPADMAWLRR CLEEKPQGQL QDIERHKLNA MGAFAEAQTC RRLVLLNYFG EGRQEPCGNC DICLDPPKQY DGSTDAQIAL STIGRVNQRF GMGYVVEVIR GANNQRIRDY GHDKLKVYGM GRDKSHEHWV SVIRQLIHLG LVTQNIAQHS ALQLTEAARP VLRGESSLQL AVPRIVALKP KAMQKSFGGN YDRKLFAKLR KLRKSIADES NVPPYVVFND ATLIEMAEQM PITASEMLSV NGVGMRKLER FGKPFMALIR AHVDGDDEE |
| 预测分子量 | 68 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RecQ重组蛋白的3-4篇参考文献及其摘要内容：
1. **文献名称**: *The Bloom's syndrome gene product is homologous to RecQ helicases*
**作者**: Ellis, N. A., et al.
**摘要**: 该研究首次发现人类Bloom综合征(BS)相关基因BLM编码的蛋白属于RecQ解旋酶家族，揭示了BLM蛋白通过维持基因组稳定性防止染色体断裂和癌症发生，为遗传性疾病与DNA修复缺陷的关联提供了关键证据。
2. **文献名称**: *RecQ helicases: caretakers of the genome*
**作者**: Hickson, I. D.
**摘要**: 文章系统总结了RecQ家族解旋酶(如BLM、WRN、RECQL4)在DNA复制、重组和修复中的核心作用，强调其通过解旋DNA二级结构、调控同源重组修复来维持基因组稳定性，并指出其缺陷导致早衰症、癌症易感性等疾病。
3. **文献名称**: *Structure and mechanism of the RecQ helicase family*
**作者**: Bernstein, D. A., & Keck, J. L.
**摘要**: 通过结构生物学分析，揭示了RecQ解旋酶的保守结构域(如解旋酶核心、HRDC结构域)与其功能的关系，探讨了其如何结合并解旋不同DNA底物(如G4四链体、霍利连接体)，为理解RecQ在基因组维护中的分子机制提供了框架。
4. **文献名称**: *Werner syndrome protein and DNA replication*
**作者**: Croteau, D. L., et al.
**摘要**: 研究阐明了Werner综合征蛋白(WRN，RecQ家族成员)在应对复制压力中的作用，包括促进停滞复制叉的修复、协调端粒维持，并指出WRN缺失导致复制错误累积和早衰表型，为靶向RecQ缺陷的癌症治疗提供了潜在策略。
以上文献涵盖了RecQ蛋白的遗传疾病关联、分子机制、结构功能及在DNA复制中的角色，反映了该领域的核心研究方向。
**Background of RecQ Recombinant Proteins**
RecQ recombinant proteins belong to the RecQ helicase family, a group of evolutionarily conserved enzymes critical for maintaining genomic stability. Named after the *E. coli* RecQ protein, these helicases are involved in DNA repair, replication, recombination, and telomere maintenance. In humans, the family includes five members: RECQL1. BLM (RECQL2), WRN (RECQL3), RECQL4. and RECQL5. Mutations in BLM, WRN, or RECQL4 are linked to genetic disorders such as Bloom syndrome, Werner syndrome, and Rothmund-Thomson syndrome, respectively, characterized by genomic instability, cancer predisposition, and premature aging.
Recombinant RecQ proteins are produced via molecular cloning and expression in systems like *E. coli* or yeast, enabling large-scale purification for functional studies. These proteins retain helicase activity, unwinding DNA structures (e.g., duplexes, Holliday junctions, G-quadruplexes) that arise during replication stress or damage. Their roles in resolving replication fork stalls, suppressing illegitimate recombination, and coordinating with other repair proteins (e.g., topoisomerases, BRCA1/2) highlight their importance in preventing carcinogenesis.
Studying recombinant RecQ variants (wild-type or disease-linked mutants) provides insights into helicase mechanisms, structure-function relationships, and disease pathogenesis. They are also tools for screening helicase inhibitors or modulators as potential anticancer therapies. Despite progress, questions remain about their regulation, tissue-specific roles, and interactions with broader DNA damage response networks. RecQ recombinant proteins thus serve as essential models for understanding genome maintenance and developing therapies for related disorders.
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