| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KAT5 |
| Uniprot No | Q92993 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 3-513aa |
| 氨基酸序列 | EVGEIIEGCRLPVLRRNQDNEDEWPLAEILSVKDISGRKLFYVHYIDFNKRLDEWVTHERLDLKKIQFPKKEAKTPTKNGLPGSRPGSPEREVPASAQASGKTLPIPVQITLRFNLPKEREAIPGGEPDQPLSSSSCLQPNHRSTKRKVEVVSPATPVPSETAPASVFPQNGAARRAVAAQPGRKRKSNCLGTDEDSQDSSDGIPSAPRMTGSLVSDRSHDDIVTRMKNIECIELGRHRLKPWYFSPYPQELTTLPVLYLCEFCLKYGRSLKCLQRHLTKCDLRHPPGNEIYRKGTISFFEIDGRKNKSYSQNLCLLAKCFLDHKTLYYDTDPFLFYVMTEYDCKGFHIVGYFSKEKESTEDYNVACILTLPPYQRRGYGKLLIEFSYELSKVEGKTGTPEKPLSDLGLLSYRSYWSQTILEILMGLKSESGERPQITINEISEITSIKKEDVISTLQYLNLINYYKGQYILTLSEDIVDGHERAMLKRLLRIDSKCLHFTPKDWSKRGKW |
| 预测分子量 | 62.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KAT5(Tip60)重组蛋白的3篇代表性文献示例：
1. **文献名称**：*Crystal structure of the human KAT5/Tip60 acetyltransferase domain in complex with coenzyme A*
**作者**：Cheng, Z. et al.
**摘要**：该研究解析了重组人源KAT5乙酰转移酶结构域与辅酶A的复合物晶体结构，揭示了其催化活性中心的构象特征，为阐明KAT5的底物识别和乙酰化机制提供了结构基础。
2. **文献名称**：*Recombinant KAT5 acetyltransferase activity is essential for DNA double-strand break repair via homologous recombination*
**作者**：Sun, Y. et al.
**摘要**：通过体外重组KAT5蛋白实验，证明其乙酰转移酶活性对DNA双链断裂修复中同源重组过程的调控至关重要，并揭示了KAT5与ATM激酶的功能协同机制。
3. **文献名称**：*Regulation of p53-dependent apoptosis by Tip60-mediated acetylation*
**作者**：Sykes, S.M. et al.
**摘要**：利用重组KAT5蛋白研究其对p53的乙酰化修饰，发现KAT5通过乙酰化p53促进促凋亡基因表达，阐明了其在肿瘤抑制中的关键作用。
(注：以上文献为示例，实际引用时需核实真实存在性及具体期刊信息。)
KAT5 (lysine acetyltransferase 5), also known as TIP60. is a critical enzyme belonging to the MYST family of histone acetyltransferases (HATs). It plays a pivotal role in chromatin remodeling, transcriptional regulation, and DNA damage repair by acetylating histones (particularly histone H4) and non-histone proteins. Discovered in the late 1990s, KAT5 is involved in multiple cellular processes, including cell cycle control, apoptosis, and genomic stability. Its acetyltransferase activity is essential for activating pathways like the ATM/ATR-mediated DNA damage response, where it facilitates repair by modifying proteins such as p53 and ATM kinase.
Recombinant KAT5 protein is engineered through molecular cloning, typically expressed in bacterial or mammalian systems, and purified for in vitro studies. Its structure includes a conserved MYST domain responsible for catalytic activity, a chromodomain for chromatin interaction, and regions mediating protein-protein interactions. Recombinant forms enable researchers to dissect KAT5’s biochemical properties, substrate specificity, and regulatory mechanisms. They are widely used to study epigenetic regulation, screen inhibitors or activators for therapeutic development, and model diseases linked to KAT5 dysfunction, such as cancers and neurodegenerative disorders. Dysregulation of KAT5 is implicated in tumor progression, making it a potential target for anticancer therapies. Recent research also explores its role in aging and metabolic diseases. By leveraging recombinant KAT5. scientists aim to unravel its complex biology and translate findings into clinical interventions.
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