| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Nr2c2 |
| Uniprot No | P49116 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 3-592aa |
| 氨基酸序列 | SPSPRIQIISTDSAVASPQRIQIVTDQQTGQKIQIVTAVDASGSPKQQFILTSPDGAGTGKVILASPETSSAKQLIFTTSDNLVPGRIQIVTDSASVERLLGKTDVQRPQVVEYCVVCGDKASGRHYGAVSCEGCKGFFKRSVRKNLTYSCRSNQDCIINKHHRNRCQFCRLKKCLEMGMKMESVQSERKPFDVQREKPSNCAASTEKIYIRKDLRSPLIATPTFVADKDGARQTGLLDPGMLVNIQQPLIREDGTVLLATDSKAETSQGALGTLANVVTSLANLSESLNNGDTSEIQPEDQSASEITRAFDTLAKALNTTDSSSSPSLADGIDTSGGGSIHVISRDQSTPIIEVEGPLLSDTHVTFKLTMPSPMPEYLNVHYICESASRLLFLSMHWARSIPAFQALGQDCNTSLVRACWNELFTLGLAQCAQVMSLSTILAAIVNHLQNSIQEDKLSGDRIKQVMEHIWKLQEFCNSMAKLDIDGYEYAYLKAIVLFSPDHPGLTSTSQIEKFQEKAQMELQDYVQKTYSEDTYRLARILVRLPALRLMSSNITEELFFTGLIGNVSIDSIIPYILKMETAEYNGQIT |
| 预测分子量 | 68.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **Nr2c2(TR4)重组蛋白** 的3篇代表性文献的模拟示例(基于研究领域常见方向整理)：
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1. **标题**: *"TR4 Nuclear Receptor Regulates Hepatic Lipogenesis Through Direct Transcriptional Activation of SREBP-1c"*
**作者**: Lee et al.
**摘要**: 研究揭示了Nr2c2重组蛋白在肝脏脂质代谢中的调控作用，通过直接激活SREBP-1c基因促进脂肪酸合成，为代谢综合征治疗提供新靶点。
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2. **标题**: *"Recombinant TR4 Protein Modulates Inflammatory Response in Macrophages via NF-κB Pathway"*
**作者**: Zhang et al.
**摘要**: 利用重组Nr2c2蛋白进行体外实验，发现其通过抑制NF-κB信号通路减轻巨噬细胞炎症反应，提示其在抗炎治疗中的潜在应用价值。
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3. **标题**: *"Structural Characterization of Human TR4 Ligand-Binding Domain and Its Interaction with Natural Agonists"*
**作者**: Wang et al.
**摘要**: 通过X射线晶体学解析了Nr2c2重组蛋白的配体结合域结构，并鉴定出内源性配体(如脂肪酸衍生物)的结合模式，为药物设计奠定结构基础。
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**注**：以上为模拟摘要，实际文献需通过PubMed/Google Scholar等平台检索关键词 **"Nr2c2 recombinant protein"** 或 **"TR4 nuclear receptor"** 获取。
Nuclear receptor subfamily 2 group C member 2 (NR2C2), also known as testicular receptor 4 (TR4), is a ligand-dependent transcription factor belonging to the nuclear receptor superfamily. It contains a conserved DNA-binding domain (DBD) and a ligand-binding domain (LBD), enabling it to regulate gene expression by binding to specific DNA response elements. NR2C2 is ubiquitously expressed but shows higher activity in reproductive tissues, the liver, and the nervous system.
Functionally, NR2C2 plays diverse roles in metabolism, embryonic development, circadian rhythm regulation, and reproductive processes. It interacts with co-regulators to modulate pathways like Wnt/β-catenin and NF-κB, influencing cell proliferation, differentiation, and apoptosis. Emerging studies link NR2C2 dysregulation to metabolic disorders, infertility, and cancers, particularly prostate and liver malignancies.
Recombinant NR2C2 protein is engineered for in vitro studies to dissect its molecular mechanisms. Produced via bacterial or mammalian expression systems, the purified protein retains structural and functional integrity, enabling DNA-binding assays, protein interaction studies, and high-throughput drug screening. Its recombinant form aids in identifying potential ligands—a critical step since endogenous ligands remain unidentified. Researchers also utilize it to explore post-translational modifications (e.g., phosphorylation) that modulate its activity.
Despite progress, challenges persist in understanding NR2C2's tissue-specific roles and ligand dependency. Recombinant protein tools are pivotal in resolving these gaps, offering insights into therapeutic targeting for diseases linked to NR2C2 pathways.
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