| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | pstS1 |
| Uniprot No | P9WGU0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-373aa |
| 氨基酸序列 | CGSKPPSGSPETGAGAGTVATTPASSPVTLAETGSTLLYPLFNLWGPAFHERYPNVTITAQGTGSGAGIAQAAAGTVNIGASDAYLSEGDMAAHKGLMNIALAISAQQVNYNLPGVSEHLKLNGKVLAAMYQGTIKTWDDPQIAALNPGVNLPGTAVVPLHRSDGSGDTFLFTQYLSKQDPEGWGKSPGFGTTVDFPAVPGALGENGNGGMVTGCAETPGCVAYIGISFLDQASQRGLGEAQLGNSSGNFLLPDAQSIQAAAAGFASKTPANQAISMIDGPAPDGYPIINYEYAIVNNRQKDAATAQTLQAFLHWAITDGNKASFLDQVHFQPLPPAVVKLSDALIATIS |
| 预测分子量 | 39.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于pstS1重组蛋白的参考文献及其摘要概括：
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1. **文献名称**: *"Evaluation of recombinant phosphate-binding protein PstS1 of Mycobacterium tuberculosis as a diagnostic marker for tuberculosis"*
**作者**: Li et al.
**摘要**: 该研究在大肠杆菌中表达并纯化了重组pstS1蛋白，评估其作为结核病血清学诊断标志物的潜力。结果显示，pstS1抗原在活动性结核患者血清中具有较高特异性抗体反应，但与卡介苗接种者存在一定交叉反应。
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2. **文献名称**: *"Cloning, expression, and immunogenicity of novel fusion protein antigens based on PstS1 (Rv0934) of Mycobacterium tuberculosis"*
**作者**: Karbalaei et al.
**摘要**: 研究通过基因工程技术将pstS1与其他结核抗原(如ESAT-6)融合表达，评估重组融合蛋白的免疫原性。实验表明，融合蛋白能诱导小鼠产生更强的Th1型细胞免疫应答，提示其在亚单位疫苗开发中的应用前景。
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3. **文献名称**: *"Biochemical characterization of the Mycobacterium tuberculosis phosphate transport system component PstS1"*
**作者**: Braibant et al.
**摘要**: 通过体外重组表达pstS1蛋白，验证其作为磷酸盐转运系统关键组分的功能。研究揭示了pstS1与磷酸盐的高亲和力结合特性，并探讨其在结核分枝杆菌环境适应中的分子机制。
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4. (可选补充)
**文献名称**: *"Improving tuberculosis diagnosis by combining recombinant PstS1 antigen with traditional smear microscopy"*
**作者**: Wang et al.
**摘要**: 提出将重组pstS1抗原的ELISA检测与传统痰涂片法联用，可显著提高结核病早期诊断的敏感性和特异性，尤其在涂片阴性病例中显示出重要辅助价值。
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这些研究聚焦于pstS1重组蛋白的表达优化、诊断应用及免疫机制解析，可作为结核病检测技术或疫苗研发的参考依据。
**Background of pstS1 Recombinant Protein**
The pstS1 recombinant protein is derived from the *pstS1* gene, which encodes a phosphate-binding transporter subunit in *Mycobacterium tuberculosis* (Mtb), the causative agent of tuberculosis (TB). This protein is part of the phosphate-specific transport (Pst) system, a critical mechanism for bacterial phosphate uptake under low-phosphate conditions. The Pst system, composed of subunits PstS, PstC, PstA, and PstB, enables Mtb to scavenge inorganic phosphate (Pi) from host environments, supporting survival and virulence during infection.
Structurally, pstS1 is a periplasmic substrate-binding protein that captures extracellular phosphate and transfers it to the transmembrane permease components (PstA/PstC) for transport into the cytoplasm. Its high-affinity phosphate-binding capability makes it essential for Mtb’s adaptation to phosphate-limited niches, such as macrophages. Beyond nutrient acquisition, studies suggest pstS1 may modulate host immune responses, potentially aiding immune evasion.
Recombinant pstS1 is produced via genetic engineering, often in *E. coli* expression systems, to study its biochemical properties, antigenicity, and role in pathogenesis. It has garnered interest as a diagnostic or vaccine candidate due to its immunogenicity in TB patients. Antibodies against pstS1 are detected in sera from active TB cases, highlighting its potential as a serodiagnostic marker. Additionally, its conservation across Mtb strains and involvement in critical metabolic pathways make it a target for novel therapeutics aimed at disrupting bacterial phosphate homeostasis.
Current research focuses on elucidating its structural-functional relationships, host interactions, and utility in TB control strategies, particularly in the context of drug-resistant TB. The protein’s recombinant form enables scalable production for immunological assays, structural studies, and preclinical evaluations. Understanding pstS1’s role in Mtb physiology and host-pathogen dynamics remains pivotal for advancing TB diagnostics, vaccines, and treatments.
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