| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | APX2 |
| Uniprot No | Q1PER6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 4-250aa |
| 氨基酸序列 | KSYPEVKEEYKKAVQRCKRKLRGLIAEKHCAPIVLRLAWHSAGTFDVKTKTGGPFGTIRHPQELAHDANNGLDIAVRLLDPIKELFPILSYADFYQLAGVVAVEITGGPEIPFHPGRLDKVEPPPEGRLPQATKGVDHLRDVFGRMGLNDKDIVALSGGHTLGRCHKERSGFEGAWTPNPLIFDNSYFKEILSGEKEGLLQLPTDKALLDDPLFLPFVEKYAADEDAFFEDYTEAHLKLSELGFADK |
| 预测分子量 | 31.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于APX2重组蛋白的3篇代表性文献名称、作者及摘要内容概括：
1. **《Heterologous expression and functional characterization of ascorbate peroxidase (APX2) from Arabidopsis thaliana in E. coli》**
- **作者**: Chen et al.
- **摘要**: 研究通过在大肠杆菌中异源表达拟南芥APX2重组蛋白，优化了表达条件并纯化出高活性酶，验证其在体外清除H₂O₂的能力，为工业化生产抗氧化酶提供基础。
2. **《Overexpression of recombinant APX2 in yeast enhances tolerance to oxidative stress》**
- **作者**: Kim & Park
- **摘要**: 将拟南芥APX2基因克隆至酵母表达系统，发现重组APX2显著提高酵母对H₂O₂和重金属胁迫的抗性，证明其在真核生物中的抗氧化应用潜力。
3. **《Characterization of a thermostable APX2 variant engineered via site-directed mutagenesis》**
- **作者**: Zhang et al.
- **摘要**: 通过定点突变改造APX2蛋白，获得耐高温重组酶(APX2-TS)，其70℃下仍保持80%活性，拓展了APX2在工业高温催化场景的应用可能性。
注：上述文献为模拟示例，实际研究中建议通过PubMed、Web of Science或Google Scholar以“APX2 recombinant protein”“APX2 heterologous expression”为关键词检索近期论文。
**Background of APX2 Recombinant Protein**
Ascorbate peroxidase 2 (APX2) is a key enzyme in the antioxidant defense system of plants, primarily involved in detoxifying reactive oxygen species (ROS) such as hydrogen peroxide (H₂O₂). As a member of the ascorbate peroxidase family, APX2 catalyzes the conversion of H₂O₂ to water using ascorbate as an electron donor, thereby mitigating oxidative stress caused by environmental stressors like high light intensity, drought, salinity, or extreme temperatures. Unlike its constitutively expressed isoform APX1. APX2 is stress-inducible and plays a critical role in protecting cellular components, particularly chloroplasts, from oxidative damage during abiotic stress.
The interest in recombinant APX2 stems from its potential applications in enhancing stress tolerance in crops and bioengineering. Recombinant APX2 is produced through heterologous expression systems, such as *Escherichia coli* or yeast, enabling large-scale purification for functional studies. Its recombinant form retains enzymatic activity, allowing researchers to explore its kinetic properties, structural stability, and interaction with other redox regulators.
Studies on APX2 have also highlighted its role in signaling pathways, linking ROS homeostasis to stress-responsive gene expression. Overexpression of APX2 in transgenic plants has shown improved resilience to oxidative stress, suggesting its utility in developing stress-tolerant crops. Additionally, recombinant APX2 is leveraged in industrial applications, including biosensors for H₂O₂ detection and enzyme-based systems for oxidative stress research.
Overall, APX2 recombinant protein serves as a vital tool for understanding plant stress responses and advancing biotechnological solutions to address agricultural and environmental challenges.
×
