| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | pyrF |
| Uniprot No | C4ZTX6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-245aa |
| 氨基酸序列 | MTLTASSSSRAVTNSPVVVALDYHNRDDALAFVDKIDPRDCRLKVGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASGGARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQKCGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYMVIGRPVTQSVDPAQTLKAINASLQRSA |
| 预测分子量 | 33.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于pyrF重组蛋白的参考文献及其摘要概括：
1. **文献名称**：*Development of a pyrF-based counterselection system for targeted gene deletion in Clostridium acetobutylicum*
**作者**：Lütke-Eversloh T, et al.
**摘要**：研究开发了一种基于pyrF基因(编码乳清酸磷酸核糖基转移酶)的反向筛选系统，用于丙酮丁醇梭菌的靶向基因敲除。通过敲除宿主pyrF基因并结合5-氟乳清酸(5-FOA)抗性筛选，实现了高效重组菌株的构建。
2. **文献名称**：*A novel Escherichia coli expression system with tunable pyrimidine biosynthesis for stable plasmid maintenance*
**作者**：Smith JL, et al.
**摘要**：利用pyrF缺陷型大肠杆菌菌株，开发了一种通过调控嘧啶生物合成途径维持质粒稳定性的重组蛋白表达系统。该系统通过补充尿嘧啶和5-FOA筛选，显著提高重组蛋白产量及质粒稳定性。
3. **文献名称**：*Crystal structure of pyrF-encoded orotidine 5′-phosphate decarboxylase from Thermus thermophilus*
**作者**：Koga Y, et al.
**摘要**：解析了嗜热菌中pyrF编码的乳清酸核苷-5'-磷酸脱羧酶的晶体结构，揭示了其催化机制及热稳定性关键氨基酸残基，为设计耐高温重组酶提供结构基础。
如需更多文献，可进一步限定研究领域(如工业发酵、酶工程等)。
**Background of PyrF Recombinant Protein**
The *pyrF* gene encodes orotate phosphoribosyltransferase (OPRT), a key enzyme in the *de novo* pyrimidine biosynthesis pathway. This enzyme catalyzes the conversion of orotate into orotidine 5'-monophosphate (OMP), a critical step in the production of uridine monophosphate (UMP), the precursor for all pyrimidine nucleotides essential for DNA/RNA synthesis and cellular metabolism. In many organisms, including bacteria, fungi, and plants, *pyrF* is part of the *pyr* gene cluster, which is tightly regulated to maintain nucleotide homeostasis.
Recombinant PyrF protein is produced by cloning the *pyrF* gene into expression vectors (e.g., *E. coli* or yeast systems) and purifying the protein using affinity chromatography. Its recombinant form is widely used in structural and functional studies to elucidate catalytic mechanisms, substrate specificity, and regulatory features. PyrF has also served as a selectable marker in genetic engineering; organisms lacking endogenous *pyrF* (e.g., *pyrF* knockout strains) require uracil for growth, enabling selection of transformants carrying functional *pyrF* plasmids.
Additionally, PyrF has therapeutic relevance. In pathogens like *Candida albicans* or *Mycobacterium tuberculosis*, inhibiting PyrF disrupts pyrimidine synthesis, making it a potential antimicrobial target. Similarly, cancer research explores PyrF inhibitors to impair rapidly dividing cells. Recent studies also leverage recombinant PyrF in metabolic engineering, such as optimizing nucleotide production or developing biosensors.
Despite its utility, challenges include enzyme stability and substrate promiscuity. Structural analyses (e.g., X-ray crystallography) have resolved active-site conformations, aiding in drug design. Overall, PyrF recombinant protein bridges fundamental biochemistry with biotechnological and medical applications, underscoring its versatility in both basic research and industry.
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