| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | atpB |
| Uniprot No | P06576 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 230-529aa |
| 氨基酸序列 | YSVFAGVGERTREGNDLYHEMIESGVINLKDATSKVALVYGQMNEPPGAR ARVALTGLTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAV GYQPTLATDMGTMQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHL DATTVLSRAIAELGIYPAVDPLDSTSRIMDPNIVGSEHYDVARGVQKILQ DYKSLQDIIAILGMDELSEEDKLTVSRARKIQRFLSQPFQVAEVFTGHMG KLVPLKETIKGFQQILAGEYDHLPEQAFYMVGPIEEAVAKADKLAEEHSS |
| 预测分子量 | 53 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于atpB重组蛋白的参考文献及其摘要概括：
1. **文献名称**: "Functional analysis of the β subunit of the ATP synthase in Escherichia coli"
**作者**: Nakamoto RK, et al.
**摘要**: 研究通过在大肠杆菌中重组表达atpB基因，证明ATP合酶β亚基对ATP水解活性的关键作用，并利用定点突变揭示了其催化位点的结构特征。
2. **文献名称**: "Chloroplast ATP synthase β subunit requires coordinated transcription and translation in plant cells"
**作者**: Fromm H, et al.
**摘要**: 在拟南芥中研究atpB基因的调控机制，发现其重组蛋白的表达依赖叶绿体与细胞核的信号互作，并通过体外重组验证β亚基对ATP合酶组装的重要性。
3. **文献名称**: "Heterologous expression and purification of the ATP synthase β subunit from spinach chloroplasts"
**作者**: McCarty DR, et al.
**摘要**: 报道了利用原核表达系统成功纯化高活性菠菜atpB重组蛋白，并证明其在重建ATP合成功能中的有效性，为酶动力学研究提供基础。
**Background of ATPB Recombinant Protein**
The *atpB* gene encodes the β-subunit of ATP synthase, a critical enzyme in cellular energy metabolism. ATP synthase catalyzes ATP synthesis during oxidative phosphorylation (in mitochondria) or photosynthesis (in chloroplasts), utilizing a proton gradient across membranes. The β-subunit plays a central role in the catalytic activity of the enzyme, directly participating in nucleotide binding and ATP formation.
Recombinant atpB protein is produced via genetic engineering, often expressed in heterologous systems like *E. coli* or yeast. This allows large-scale production of the purified protein for functional and structural studies. Research on recombinant atpB has advanced understanding of ATP synthase assembly, enzymatic mechanisms, and its regulation under varying physiological conditions.
In plant sciences, *atpB* is chloroplast-encoded, making it a model for studying organelle gene expression and photosynthesis. Its recombinant form aids in exploring mutations affecting photosynthetic efficiency or stress responses. In pathogenic bacteria, ATP synthase is a potential drug target; recombinant atpB facilitates inhibitor screening and antimicrobial development.
Additionally, recombinant atpB serves as an antigen for antibody production, enabling diagnostic tools for diseases linked to ATP synthase dysfunction. Its conserved nature across species also supports evolutionary studies of bioenergetic systems. Overall, recombinant atpB is a versatile tool bridging fundamental research and biotechnological applications.
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