| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPA17 |
| Uniprot No | Q15506 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-146aa |
| 氨基酸序列 | MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFESLLEKREKTNFDPAEWGSKVEDRFYNNHAFEEQEPPEKSDPKQEESQISGKEEETSVTILDSSEEDKEKEEVAAVKIQAAFRGHIAREEAKKMKTNSLQNEE |
| 预测分子量 | 43.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于SPA17重组蛋白的模拟参考文献(基于公开研究背景概括，非真实文献)：
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1. **文献名称**：*SPA17重组蛋白的克隆表达及其在卵巢癌中的免疫原性分析*
**作者**：Lalani, E.N. 等
**摘要**：研究通过大肠杆菌系统重组表达SPA17蛋白，证实其在卵巢癌细胞表面异常高表达，可诱导特异性T细胞免疫反应，提示其作为肿瘤疫苗靶点的潜力。
2. **文献名称**：*重组SPA17蛋白促进精子-卵子结合机制的体外研究*
**作者**：Suri, A. 等
**摘要**：利用真核表达系统获得功能性SPA17重组蛋白，发现其通过结合透明带蛋白ZP3调控精子与卵母细胞的黏附过程，为不孕症治疗提供新靶点。
3. **文献名称**：*SPA17作为癌症睾丸抗原在多种肿瘤中的表位鉴定*
**作者**：Chiang, C.L. 等
**摘要**：通过重组SPA17蛋白筛选出HLA-A2限制性CTL表位，并验证其在肺癌、乳腺癌等肿瘤组织中特异性表达，支持其用于过继性免疫治疗开发。
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注：以上内容为领域知识模拟，具体文献需通过学术数据库检索。
SPA17 (Sperm Autoantigenic Protein 17), also known as SP17. is a protein initially identified as a sperm-specific antigen involved in mammalian fertilization. It belongs to the cancer/testis antigen (CTA) family, which is characterized by restricted expression in normal tissues (primarily testes and placenta) but aberrant activation in various cancers. The human SPA17 gene is located on chromosome 11q24.2 and encodes a 151-amino acid protein with a conserved N-terminal heparin-binding domain and a C-terminal region homologous to the cysteine-rich domain of thrombospondin type-1 repeats (TSR).
Biologically, SPA17 plays roles in sperm-egg interaction by binding zona pellucida glycoproteins during fertilization. Beyond reproductive biology, it has gained attention in oncology due to its ectopic expression in malignancies like multiple myeloma, ovarian cancer, breast cancer, and hepatocellular carcinoma. This tumor-associated expression, coupled with its immunogenic properties, positions SPA17 as a potential biomarker and therapeutic target.
Recombinant SPA17 protein is engineered using expression systems (e.g., E. coli or mammalian cells) for functional studies and clinical applications. Its recombinant form enables research into molecular mechanisms in carcinogenesis, including promoting cell proliferation, migration, and immune evasion. Additionally, it serves as an antigen source for developing cancer vaccines or antibody-based therapies. Recent studies also explore its role in modulating immune responses via interactions with extracellular matrix components and immune receptors.
Ongoing research focuses on understanding its dual roles in reproduction and oncopathology, as well as optimizing recombinant SPA17 for diagnostic and immunotherapeutic strategies.
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