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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Ccl7 |
| Uniprot No | P80098 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-99aa |
| 氨基酸序列 | QPVGINTSTT CCYRFINKKI PKQRLESYRR TTSSHCPREA VIFKTKLDKE ICADPTQKWV QDFMKHLDKK TQTPKL |
| 预测分子量 | 9.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCL7重组蛋白的3篇参考文献示例：
1. **"Chemokine CCL7/MCP-3 regulates dendritic cell migration and polarization"**
- 作者：Menten P et al.
- 摘要：研究重组CCL7蛋白对树突状细胞迁移和功能极化的影响，发现其通过CCR1/CCR2受体激活ERK信号通路，促进Th1型免疫应答。
2. **"Recombinant CCL7 modulates monocyte recruitment in a murine model of acute inflammation"**
- 作者：Sozzani S et al.
- 摘要：通过重组CCL7蛋白在小鼠急性炎症模型中验证其趋化单核细胞的作用，证明其通过增强整合素表达促进单核细胞向炎症部位浸润。
3. **"CCL7 promotes breast cancer metastasis via Akt-dependent EMT pathway"**
- 作者：Zhang Y et al.
- 摘要：利用重组CCL7蛋白处理乳腺癌细胞，发现其通过激活Akt信号诱导上皮-间质转化(EMT)，进而增强肿瘤细胞的侵袭和转移能力。
注：以上文献信息为示例，实际引用需以具体发表的论文内容为准。
Ccl7 (C-C motif chemokine ligand 7), also known as monocyte chemoattractant protein-3 (MCP-3), is a small secreted protein belonging to the CC chemokine family. It plays a critical role in immune regulation by recruiting leukocytes, including monocytes, eosinophils, dendritic cells, and lymphocytes, to sites of inflammation or injury. Ccl7 exerts its biological effects by binding to G-protein-coupled receptors, primarily CCR1. CCR2. and CCR3. which are expressed on the surface of target cells. This interaction activates intracellular signaling pathways, leading to cell migration, immune response modulation, and tissue repair processes.
Recombinant Ccl7 protein is produced using engineered expression systems (e.g., *E. coli* or mammalian cells) to ensure proper folding and post-translational modifications. It typically retains the functional properties of native Ccl7. including chemotactic activity and receptor binding specificity. Structurally, Ccl7 contains a conserved N-terminal domain and a C-terminal α-helix, stabilized by two disulfide bonds critical for its three-dimensional conformation. Recombinant versions often include tags (e.g., His-tag) for purification and detection.
In research, recombinant Ccl7 is widely used to study chemokine-mediated immune responses, inflammatory diseases (e.g., asthma, rheumatoid arthritis), and cancer biology. It has been implicated in tumor microenvironment regulation, where it may promote angiogenesis and metastasis by recruiting immunosuppressive cells. Additionally, it serves as a tool for drug discovery, enabling the screening of antagonists targeting Ccl7 or its receptors. Its role as a biomarker in disease models and diagnostic applications (e.g., ELISA standards) further underscores its versatility. However, functional variability due to expression systems or tags requires careful validation in experimental settings.
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