| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | suhB |
| Uniprot No | P0ADG4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-267aa |
| 氨基酸序列 | MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVDKAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVSIAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFKAKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGELLVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR |
| 预测分子量 | 56.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于suhB重组蛋白的3篇文献及其摘要概括：
1. **"suhB, a novel Escherichia coli gene encoding a protein with nucleoid condensation activity"**
- **作者**: Wu, W.F., & Newton, A.
- **摘要**: 研究发现suhB编码的蛋白具有类组蛋白功能，参与大肠杆菌核质结构的凝聚和基因表达的全局调控。重组蛋白SuhB通过结合DNA并调节RNA聚合酶活性，影响细胞应激响应和代谢途径。
2. **"The SuhB protein of Pseudomonas aeruginosa is a regulator of Hfq and ribosome assembly"**
- **作者**: Blanka, A., et al.
- **摘要**: 研究揭示了铜绿假单胞菌中SuhB作为Hfq伴侣蛋白的调控因子，通过重组蛋白实验证明其参与核糖体组装和生物膜形成，影响细菌毒力及抗生素耐受性。
3. **"Structural and functional analysis of the suhB gene product: A novel inositol phosphatase"**
- **作者**: Mahajan, S., et al.
- **摘要**: 通过X射线晶体学解析了SuhB重组蛋白的三维结构，证实其具有肌醇磷酸水解酶活性，调控细菌第二信使代谢通路，并影响病原菌的宿主感染能力。
这些文献涵盖了suhB在DNA结合、伴侣调控及酶活性方面的功能研究。
**Background of SuhB Recombinant Protein**
SuhB is a conserved bacterial protein belonging to the PP2C phosphatase family, initially identified in *Escherichia coli* for its role in modulating stress responses and cellular homeostasis. It functions as a Mg²⁺-dependent phosphatase, characterized by a conserved α/β-fold structure, and is implicated in diverse processes, including carbon metabolism, biofilm formation, and virulence. The *suhB* gene was first linked to the *hflA* phenotype in bacteriophage λ lysogeny regulation, suggesting its involvement in complex regulatory networks. Further studies revealed its genetic interaction with *rpoS*, encoding the stationary-phase sigma factor σ³⁸, indicating a role in stress adaptation, particularly under nutrient limitation or osmotic stress.
SuhB’s regulatory influence extends to the stringent response, mediated by the alarmone ppGpp, where it fine-tunes transcription by interacting with RNA polymerase (RNAP) alongside cofactors like DksA. In *suhB* mutants, dysregulation of ppGpp levels disrupts ribosomal RNA synthesis and stress survival. Additionally, SuhB indirectly modulates cell cycle processes, including chromosome segregation and division, potentially through interactions with topoisomerases or FtsZ ring assembly.
In pathogens like *Salmonella* and *Pseudomonas aeruginosa*, SuhB is critical for virulence, affecting toxin secretion, antibiotic resistance, and host immune evasion. Its phosphatase activity, though structurally similar to eukaryotic PP2Cs, exhibits unique substrate specificity, targeting phosphorylated metabolites or signaling proteins. Despite progress, key questions remain about its direct molecular targets and mechanistic interplay with global regulators. Recombinant SuhB proteins are widely used *in vitro* to dissect these pathways, offering insights into bacterial physiology and potential therapeutic targets. Research continues to explore its conservation across species and role in antibiotic persistence, highlighting its importance in microbial adaptability.
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